梗阻性结直肠癌中E-cadherin、CD44v6的表达及与预后的关系  

Expression of E-cadherin and CD44v6 in human obstructive colorectal cancer:correlation with prognosis

作　　者：赵鹏[1] 白鍊 李启刚[1] 温泽霖 简斌[1] 李中福[1] 吴帅[1] 谢建[1] 廖娟[2] 钟正福[3] 

机构地区：[1]重庆医科大学附属永川医院普外科,重庆402160 [2]重庆医科大学附属永川医院中心实验室,重庆402160 [3]重庆医科大学附属永川医院病理科,重庆402160

出　　处：《第三军医大学学报》2016年第6期629-633,共5页Journal of Third Military Medical University

基　　金：重庆医科大学附属永川医院青年科研项目(YJQN20120022)~~

摘　　要：目的探讨梗阻性结直肠癌中E-cadherin(E-cad)、CD44v6的表达及与预后的关系。方法采用免疫组织化学方法分别检测48例梗阻性结直肠癌、121例非梗阻性结直肠癌组织和30例癌旁肠黏膜组织中E-cad和CD44v6蛋白的表达,Kaplan-Meier法绘制生存曲线,Cox比例风险模型多因素分析。结果梗阻性结直肠癌组织中E-cad和CD44v6的标准化阳性率分别为30.2%和81.3%,非梗阻性结直肠癌组织中E-cad和CD44v6的标准化阳性率分别为51.8%和58.9%,两组差异有统计学意义(P<0.05)。E-cad的表达与肿瘤p TNM分期、淋巴结转移、分化程度、浸润深度有关(P<0.05)。CD44v6表达与肿瘤p TNM分期和淋巴结转移有关(P<0.05)。单因素生存分析显示:梗阻、E-cad和CD44v6表达是影响预后的危险因素。Cox回归模型显示:E-cad的表达、淋巴结转移、分化程度为影响结直肠癌患者预后的独立危险因素。结论 E-cad表达下降和CD44v6表达升高与梗阻性结直肠癌较差的预后密切相关。Objective To investigate the expression of E-cadherin （E-cad） and CD44v6 in human obstructive colorectal cancer and their relationships with prognosis. Methods Forty-eight cases of obstructive colorectal cancer, 121 cases of non-obstructive colorectal cancer, and 30 cases of adjacent non-tumor tissues were selected for immunohistochemical staining of E-cad and CI）44v6 proteins. Survival curves were described with Kaplan-Meier analysis. Multivariate analysis was performed with Cox proportional hazard model. Results There were significant differences in the standardized positive rates of E-cad and CD44v6 between obstructive colorectal cancer group and non-obstructive colorectal cancer group （30.2% vs 51.8%, 81.3% vs 58.9%, P 〈0.05）. The expression of E-cad was correlated to pTNM stage, node involvement, histological grade and depth of invasion （P 〈 0.05）. The expression of CD44v6 was correlated to pTNM stage and node involvement. Univariate survival analysis showed that obstruction and expression of CD44v6 and E-cad were the risk factors for prognosis. Cox multivariate analysis showed that the expression of E-cad, node involvement and histological grade were the independent risk factors for the prognosis of patients with Conclusion Down-regulated expression of E-cad and up-regulated expression of CD44v6 to poor prognosis of the patients with obstructive colorectal cancer. colorectal cancer. are closely related

关 键 词：梗阻性结直肠癌 CD44V6 E-CADHERIN 上皮间质转化 

分 类 号：R446.8[医药卫生—诊断学] R730.23[医药卫生—临床医学]