右美托咪啶对脓毒症急性肺损伤大鼠IL-10表达的干预作用  

Dexmedetomidine intervention effects on the expression of IL-10 in pulmonary tissue of rats with sepsis-associated acute lung injury

作　　者：陈璐[1] 冯伟[1] 郭轶男[1] 

机构地区：[1]沈阳市第四人民医院重症医学科,辽宁沈阳110031

出　　处：《解剖学研究》2016年第1期16-18,24,共4页Anatomy Research

基　　金：辽宁省教育厅科研基金(L2010687)

摘　　要：目的研究脓毒症大鼠肺组织白介素-10(IL-10)表达的变化规律,探讨右美托咪啶对对脓毒症大鼠肺组织的保护作用及可能机制。方法雄性SD大鼠75只,随机分为3组:假手术组、脓毒症模型组和右美托咪啶组,每组25只,检测肺组织湿/干质量比(W/D),免疫组织化学方法和Western blot方法检测肺组织IL-10蛋白的表达。结果与假手术相比,急性肺损伤组的W/D比值显著升高(P<0.05),应用右美托咪啶治疗后,W/D比值显著降低(P<0.05)。与对照组相比,急性肺损伤组大鼠肺组织IL-10蛋白表达的平均光密度值显著降低(P<0.05);与急性肺损伤组相比,右美托咪啶治疗组大鼠肺组织IL-10蛋白表达的平均光密度值显著升高(P<0.05)。结论右美托咪啶能够通过上调抗炎因子IL-10的表达,从而减轻脓毒症大鼠的肺损伤,Objective To study the regulation of IL-10 change in the lung tissue of rats with sepsis-associated acute lung injury（ALI）,and to investigate the dexmedetomidine protective effect on lung of rats with sepsis-associated ALI. Methods 75 male Sprague-Dawley（SD）rats were divided into sham operation group,sepsis model group and dexmedetomidine group,25 rats in each group. The lung wet/dry（W/D）weight ratio was detected,and The expression of IL-10 in lung tissue of rats in each group were detected by immunohistochemistry method and Western blot method. Results Compared with sham operation group,the W/D ratio of rats in ALI group was higher（P〈0.05）,however,the W/D ratio of rats in dexmedetomidin group was decreased significantly after dexmedetomidin treatment（P〈0.05）. Compared with sham operation group,the mean optical density（MOD）value of IL-10 positive expressions in lung tissue of rats in sepsis model group was lower. Compared with sepsis model group,the MOD value of IL-10 positive expressions in lung tissue of rats in dexmedetomidin group was increased significantly（P〈0.05）. Conclusion Dexmedetomidine could attenuate lung inflammatory reaction in septic rats by up-regulating the expression of IL-10.

关 键 词：右美托咪啶 脓毒症 急性肺损伤 白介素-10 大鼠 

分 类 号：R459.7[医药卫生—急诊医学]