丙烯酰胺染毒对乳鼠海马齿状回神经元SYP和DCX表达的影响  

Effects of acrylamide exposure on the expression of SYP and DCX in the hippocampal dentate gyrus neurons in offspring rats

作　　者：杨德慧[1] 李细霞[1] 赵萌萌[1] 张亚琼[1] 李国营[1] 马宇昕[1] 罗利[1] 刘靖[1] 

机构地区：[1]广东药学院基础学院人体解剖学教研室,广东广州510006

出　　处：《解剖学研究》2016年第1期25-27,35,共4页Anatomy Research

摘　　要：目的探究丙烯酰胺(acrylamide,ACR)孕哺期染毒对乳鼠海马齿状回神经元突触囊泡膜蛋白(SYP)和微管相关蛋白(DCX)表达的影响。方法 32只SPF级SD雌鼠随机分为对照组(0 ppm)和低(50 ppm)、中(100 ppm)、高(200 ppm)剂量染毒组。在雌鼠妊娠第6天至分娩后第21天,通过饮水建立ACR母源性染毒模型。乳鼠出生后第21天取材,免疫荧光组织化学检测海马神经元SYP和DCX的表达情况。结果免疫荧光组织化学检测结果显示,与对照组和低剂量组相比较,中、高剂量组海马齿状回中SYP和DCX阳性细胞的平均光密度均显著减小(P<0.05)。结论 ACR染毒可能是通过降低海马齿状回神经元SYP和DCX的表达,干扰海马神经元突触的形成及神经元的增殖和分化,抑制神经元的发育。Objective To study effects of acrylamide（ACR）exposure on the expression of Synaptophysin（SYP）and Doublecortin（DCX）of neuron in the hippocampal dentate gyrus neurons in offspring rats. Methods On gestational day 6,dams were randomly divided into four groups of eight dams each and given ACR at 0,50,100 or 200 ppm in their drinking water from gestational day 6 to postnatal day 21. On postnatal day 21,all the offspring were killed and immunofluorescence administered. Results Immunofluorescence staining showed that SYP positive cells of 100 ppm or 200 ppm groups significant decrease in the hippocampal dentate gyrus as compared with 0 ppm control or 50 ppm groups,as well as DCX（P〈0.05）. Conclusion The decreases of SYP and DCX levels after maternal exposure to ACR,which might impaired the formation of synapses and decreased neuronal proliferation and migration in the hippocampal dentate gyrus,inhibited the development of neurons。

关 键 词：丙烯酰胺 海马齿状回 神经元发育 突触囊沧膜蛋白(SYP) 微管相关蛋白(DCX) 

分 类 号：R114[医药卫生—卫生毒理学]