阿片生长因子Met-ENK对多种肿瘤细胞的生长抑制作用研究  

Study on the inhibition effect of methionine enkephalin on the growth of a variety of tumor cells

作　　者：杨峥维[1] 李彦博[2] 樊春艳[1] 

机构地区：[1]海军总医院药剂科,北京100048 [2]军事医学科学院附属医院医务部,北京100071

出　　处：《中国临床药理学与治疗学》2016年第2期130-133,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(81372251)

摘　　要：目的:研究阿片生长因子Met-ENK(methionine enkephalin,Met-ENK)对多种肿瘤细胞的抑制作用,分析其对不同肿瘤细胞系的抑制作用差异性及特点。方法:采用CCK-8法考察不同浓度Met-ENK对SH-SY5Y细胞的增殖抑制活性;采用细胞计数法计数相同浓度Met-ENK作用下,SHSY5Y细胞随时间的生长曲线;采用CCK-8法检测不同浓度的Met-ENK对8种不同的肿瘤细胞系细胞的抑制作用。结果:不同浓度的Met-ENK对SH-SY5Y细胞具有不同的抑制作用,IC50为5.1×10^(-5)mol/L;Met-ENK作用后SH-SY5Y在48 h生长抑制明显(P<0.01);不同浓度的MetENK对8种不同的肿瘤细胞系细胞均有抑制作用,其中对SMCC-7721抑制作用最为明显。结论:Met-ENK具有广谱抗肿瘤作用,但抑制强度不同,具有时间依赖性,其对8种不同的肿瘤细胞系细胞均有不同强度的抑制作用。AIM： To study the inhibition effect of Met-ENK on a wide variety of tumor cells,analyzing its differences of inhibition effect on different tumor cell lines. METHODS： CCK 8 was used to investigate the different concentrations of Met ENK to inhibit the growth of SH SY5Y. Count method was used to draw the curve that the SH-SY5Y growth inhibition as changes over time under the same concentration. Different concentrations of Met-ENK were adopted on eight kinds of tumor cells by CCK 8 method. RESULTS： The inhibition effects of different concentrations of Met-ENK on SH-SY5Y cell line were different. At different time points especially in 48 hours Met-ENK obviously inhibited the growth of SH-SY5Y（ P 〈0. 01）. Different concentrations of Met-ENK had inhibitory effects on eight tumor cells especially SMCC-7721. CONCLUSION： Met-ENK has broad-spectrum inhibition effect on tumor cells,the inhibitory effect is timedependent. Met-ENK has inhibition effects on eight different tumor cell lines with various degrees.

关 键 词：Met-ENK SH-SY5Y 肿瘤生长抑制 广谱作用 

分 类 号：R965.2[医药卫生—药理学]