右美托咪定对小鼠肺缺血-再灌注损伤的保护作用  

Protection of Dexmedetomidine on the lung ischemia-reperfusion injury in mice

作　　者：郭长满[1] 陈丹[2] 宋东 王万铁[2] 邱晓晓 

机构地区：[1]温州医科大学环境与公共卫生学院 [2]温州医科大学病理生理学教研室,浙江温州325000

出　　处：《中国临床药理学与治疗学》2016年第2期145-149,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：浙江省公益性技术应用研究计划(2013C33168);浙江省大学生科技创新项目(省新苗人才计划)(2013R413043);温州市科技计划项目(Y20140004)

摘　　要：目的：探讨右美托咪定对小鼠肺缺血/再灌注损伤的保护作用。方法：实验用雄性8～10周龄C57BL/6J小鼠50只,随机分为5组：假手术组（sham operation,sham组）,缺血-再灌注组（I/R组）,I/R＋生理盐水组（NS组）,I/R＋右美托咪定组（DEX组）,I/R＋阿替美唑（Atipamezole,ATI）＋DEX组（ATI＋DEX组）。复制在体小鼠左肺I/R损伤模型,I/R组小鼠开胸夹闭左肺门缺血30 min,再灌注180 min;sham组仅开胸游离肺门而不夹闭,肺门游离后机械通气210 min;DEX组在左肺缺血前30 min腹腔注射DEX25μg/kg,NS组则腹腔注射同体积生理盐水;ATI＋DEX组在缺血前30 min腹腔注射ATI250μg/kg后,随即腹腔注射DEX 25μg/kg。实验结束时取左肺组织测肺组织湿/干重比（W/D）、总肺含水量（TLW）,评估肺组织损伤指数（IQA）,光镜和电镜下观察肺组织的形态学变化。结果：与sham组比,其余4组肺组织W/D、TLW、IQA值明显上升（P〈0.05）,光镜、电镜示肺组织呈明显损伤性变化;I/R组与NS组间上述指标差异无统计学意义（P〉0.05）;DEX干预后,肺组织W/D、TLW、IQA值较I/R组明显降低（P〈0.01）,肺组织损伤明显改善;应用阿替美唑以后,肺组织损伤加重,W/D、TLW、IQA值明显升高,与DEX组相比差异具有统计学意义（P〈0.01）。结论：右美托咪定可能通过抑制交感活性,改善缺血区域的血流,对缺血再灌注肺发挥保护作用。AIM： To explore the protection of Dexmedetomidine（ DEX） on the lung ischemiareperfusion injury in mice. METHODS： 50C57 BL /6J male mice,aged 8-10 weeks,were randomly divided into 5 groups（ n = 10） ： sham operation group（ sham group）,lung ischemia-reperfusion group（ I/R group）,I/R ＋ saline（ NS group）,I/R＋ DEX group（ DEX group）,I/R ＋ ATI ＋ DEX group（ ATI ＋ DEX group）. The lung ischemiareperfusion injury model in mice was replicated. In I/R group,the hilum of left lung was clamped for 30 min,followed by 180 min-reperfusion. For sham group,the hilum of left lung was only separated without clamping. DEX（ 25 μg/kg） was infused intraperitoneally（ i. p） 30 min prior to clamping in DEX group,NS group was infused（ i. p） with the same volume normal saline,then followed by 180min-reperfusion period. ATI ＋ DEX group was infused intraperitoneally（ i. p） with ATI（ 250 μg/kg,i. p.） prior to DEX infusion. After reperfusion,the left lung tissues were harvested to determine the lung wet/dry weight（ W/D）,the total lung water content（ TLW）,and index of quantitative evaluation for alveolar damage（ IQA）. The morphological changes of lung tissue were observed under light microscope and electronmicroscope. RESULTS： Compared with the sham group,the values of W/D,TLW,IQA were obviously raised（ P 〈0. 05）,light microscope and electron microscope showed serious morphological and ultrastructure injury in the other four groups;the above index in I/R group and NS group showed no obvious differences（ P 〈0. 05）; compared with the I/R group,the values of W/D,TLW,IQA were significantly decreased in the DEX group（ P 〈0. 01）,meanwhile,light morphological and ultrastructure injury was found to be alleviated; in ATI ＋DEX group,the lung injury became more aggravated than that in the DEX group,the values of W/D,TLW,IQA were significantly higher than those in the DEX group. CONCLUSION： DEX can protect lung tissue from ischemia-reperfusion injury,which ma

关 键 词：右美托咪定 缺血再灌注 肺损伤 

分 类 号：R965.2[医药卫生—药理学]