妊娠期麻醉药物七氟烷暴露对出生后大鼠学习记忆的影响  

Exposure to sevoflurane during pregnancy and its effect on offspring rats

作　　者：周雪[1] 陈晓辉[2] 陆迪涵[1] 冯霞[1] 

机构地区：[1]中山大学附属第一医院麻醉科,广州510080 [2]福建省立医院麻醉科,福州350001

出　　处：《中华实验外科杂志》2016年第3期682-684,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81571032）

摘　　要：目的观察孕鼠在孕中晚期暴露不同浓度及次数七氟烷对其后出生幼鼠学习记忆的影响。方法妊娠中晚期SD乳鼠50只，建立吸入麻醉药物七氟烷暴露模型，将孕鼠随机分为5组：孕14d大鼠暴露空气对照组（C组），孕14d大鼠单次暴露0．3％七氟烷组（S1组），孕14d大鼠单次暴露1．3％七氟烷组（S2组），孕14d大鼠单次暴露2．3％七氟烷组（S3组），孕鼠于孕7、10、14d多次暴露2．3％七氟烷组（S4组）。运用Morris水迷宫试验于幼鼠出生后28d行学习记忆检测。结果Morris水迷宫试验定位航行试验各组平均逃避潜伏期随测试天数逐渐缩短，但5组每日平均逃避潜伏期差异均无统计学意义（P〉0．05），Morris水迷宫试验记忆成绩结果：统计大鼠空间平台搜索试验时的朝向角[5组依次为（22．12±8．65）。、（32．17±10．76）°、（28．33±9．15）°、（31．14±9．06）°、（27．98±11．73）°]、经过平台次数[5组依次为（4．21±1．35）、（3．46±1．67）、（3．55±1．02）、（3．22±1．04）、（3．01±2．19）次]及跨越目标象限占整个游泳距离百分率[5组依次为（43．01±10．28）％、（38．15±9．76）％、（37．11±6．81）％、（39．64±8．09）％、（36．09±7．49）％]，5组比较差异均无统计学意义（P〉0．05）。结论本实验建立孕鼠吸入麻醉药物七氟烷暴露模型，妊娠期七氟烷暴露对幼鼠产生长期的学习记忆行为学无明显影响。Objective To discuss the exposure of pregnant rats in middle or late gestation to dif- ferent concentrations and frequency of sevoflurane and the effects on their offsprings＇ learning and memory function. Methods Fifty pregnant SD rats were randomly divided into 5 groups ： control group （ pregnant D14, air exposure）, sevoflurane group 1 （pregnant D14, single exposure to 0. 3% sevoflurane）, sevoflu- rane group 2 （pregnant D14, single exposure to 1.3% sevoflurane）, sevoflurane group 3 （pregnant D14, single exposure to 2. 3% sevoflurane）, and sevoflurane group 4 （ pregnant D7, D10, D14, triple exposure to 2. 3% sevoflurane）. The Morris water maze （MWM） was used to assess the cognitive learning ability of the young rats. Results The average escape latency of MWM was shortened by days, but there were no significant differences among the five groups （P 〉 0.05）. For MWM＇ s memory test, we calculated the di- rection angle [ （22. 12 ± 8.65）°, （32. 17 ± 10. 76）°, （28.33 ± 9. 15）°, （31.14 ± 9.06）°, （27.98 ± 11.73）°], times crossing the platform [（4.21 ±1.35）, （3.46±1.67）, （3.55 ±1.02）, （3.22 ± 1.04）, （3.01 ± 2. 19） times] and the percentage of distance crossing the target quadrant in the total swimming period [（43.01 ±10.28）%, （38.15 ±9.76）%, （37.11 ±6.81）%, （39.64±8.09）%, （ 36.09 ± 7.49） % ] , finding that there were also no significant differences among the five groups （ P 〉 O. 05 ）. Conclusion Our experiments set up the sevoflurane expose model of pregnant rats. The results showed that seveflurane exposure during pregnancy would not have long - term effects on learning and mem- ory function of the offspring.

关 键 词：七氟烷 孕鼠 发育脑 学习记忆 

分 类 号：R614.21[医药卫生—麻醉学]