小鼠液压冲击脑损伤模型的建立和分级  

Establishment of graded fluid percussion impact traumatic brain injury in mice

作　　者：金卫篷[1] 周源[2] 尉辉杰[2] 王东[2] 林云鹏[2] 江荣才[2] 李少武[3] 张建宁[2] 

机构地区：[1]天津医科大学第二医院神经外科,300211 [2]天津医科大学总医院神经外科,300052 [3]首都医科大学附属天坛医院北京市神经外科研究所,北京100050

出　　处：《中华实验外科杂志》2016年第3期828-832,共5页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81271361）;国家自然科学青年科学基金（81100920）;天津市应用基础及前沿技术研究计划（12JCQNJC07200、12JCQNJC06800）

摘　　要：目的建立稳定的小同级别小鼠液压冲击颅呐损伤模型。方法采用60～80、j60～180、260～280kPa，3种小同的液胝冲击力建立小鼠液压冲击脑损伤模型。造模后进行生命体征观察；造模后第12小时、第1、2、3、7大进行改良神经功能缺损评分（mNSS）；造模后第1、3、7天行大体标本观测、2，3，5-氯化三苯基叫氮唑（TTC）染色、苏小素-伊红（HE）染色和小动物核磁共振成像（MRI）检食；造模后第7～12天行Morris水迷宫实验。结果各组小鼠目标象限再分率分别为：对照组（31．2±8．0）％，轻型损伤组（30．0±11．7）％，中型损伤组（26．4±17．4）％，重型损伤组（15．0±9．0）％，重型士员伤绀小鼠目标象限百分率与对照组小鼠目标象限自分牢比较差异有统计学意义]（15．0±9．0）％比（31．2±8．0）％，P〈0．05]。打击力度越大，小鼠脑损伤越蕾，死亡率越离，mNSS评分越高，脑水肿越重，脑拌裂伤灶和MRI影像学改变越明显。结论本文验模型可以稳定地建立小同程度小鼠颅脑创伤。Objective To establish a stable varying degree of fluid percussion impact tramnatic brain injury7 （TBI） model in mice for the future studies of traumatic brain iujury. Methods The hydraulic combat pressures for mihl injury, moderate injury and heavy injury groups were 60 -80, 160 - 180, and 260 - 280 kPa. The modified Neurological Severity Score （mNSS） test was conducted at 12 h, 1,2,3 and 7 d after TB1; 2, 3, 5- triphenyltetrazolium chloride （TTC） staining, hematoxylin- eosin （HE） staining, and the small animal Magnetic Resonance hnaging （MRI） examination were conducted at 1,3,7 d after TBI ; Morris water maze test was conducted at 7 - 12 d after TBI. Results The target quadrant percentage of sham, mild injury, moderate injury and heavy injury groups was （ 31.2 ± 8.0） % , （ 30. 0 ± 11.7 ） % , （ 26. 4 ± 17.4 ） % , （ 15.0 ± 9.0） % respectively. The target quadrant percentage of heavy injm7 group was statistically significant difference compared with the target quadrant percentage of sham group [ （ 15.0 ± 9.0） % than （ 3 1. 2 ± 8.0） % , P 〈 0. 05 ]. We observed the mice after TBI, found that the more severely brain was damaged, the higher mortality of mice, lower behavior scores, more severely brain edema and more obviously of changes in MR1 images. Conclusion This animal model can establish a stable varying degrees of fluid percussion impact traumatic brain injury model in mice, which provides an excellent animal experimental platform tor the future study of traumatic brain injury.

关 键 词：创伤性颅脑损伤 动物模型 液压冲击 

分 类 号：R651.15[医药卫生—外科学]