不结球白菜种质资源对TuMV的抗性鉴定与评价  

Identification and Evaluation of Resistance to TuMV of Brassica campestris L. ssp. chinensis Germplasm Resources

作　　者：王晓伟[1] 李锡香[1] 张晓辉[1] 邱杨[1] 董洪霞[1] 宋江萍[1] 王海平[1] 沈镝[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《植物遗传资源学报》2016年第2期387-394,共8页Journal of Plant Genetic Resources

基　　金："十二五"农村领域国家科技计划课题(2013BAD01B04-2);农业部园艺作物生物学与种质创制重点实验室项目

摘　　要：分别利用苗期人工接种鉴定及ELISA检测方法,对127份不结球白菜种质资源进行芜菁花叶病毒（TuMV）的抗性鉴定。结果显示,苗期人工接种鉴定的病情指数（DI）分布在3.55~95.68之间,不同种质间表现出较大的抗性差异。不同抗性级别的次数分布图基本符合正态分布,略向感病区域偏离。基于病情指数的聚类分析结果与抗病性分级基本一致。通过苗期鉴定共筛选获得高抗TuMV的不结球白菜种质6份,抗病种质13份,其主要农艺性状表现出一定的多样性。其中叶面皱缩和具有刺毛的抗病材料所占比例较高,可能与TuMV抗性存在一定的相关性。ELISA检测结果显示,117份供试种质的P/N值分布在3.10~25.37之间,不同种质间表现出病毒含量的差异,但与DI值未呈现明显相关性,可作为抗病材料筛选的辅助指标。Identification of the resistance to TuMV of 127 Brassica campestris L. ssp. chinensis germplasm was conducted with the method of artificial inoculation identification and ELISA test respectively. The result showed that the DI value ranged between 3. 55 and 95. 68,which expressed significant difference of resistance to TuMV among accessions. Distribution plot for different resistance level to TuMV basically corresponds to normal distribution and the peak was slightly partial to susceptible region. The result of cluster analysis based on DI value was similar to that of the grading levels. 6 accessions highly resistant to TuMV and 13 accessions resistant to TuMV were screened by seedlings inoculation identification. Their main agronomic characters demonstrated abundant diversity. The number of resistance accessions with the characters of having wrinkling or pubescence on leaf surface taken a relatively higher ratio,which demonstrated that the leaf character might have a certain correlation with TuMV resistance. The result of ELISA test showed that the amount of virus in 117 materials was significantly different with P / N value ranging between 3. 10 and 25. 37,but it had no significant correlation with DI value. ELISA test could be used as a supplementary identification method in screening resistance materials.

关 键 词：不结球白菜 TuMV抗性 ELISA检测 

分 类 号：S634.3[农业科学—蔬菜学]