网膜素改善氧化应激对人动脉血管平滑肌细胞Ⅰ、Ⅳ型胶原表达的抑制作用  

Omentin Attenues Oxidative Stress-induced Down-regulation of Ⅰ/Ⅳ Collagen in the Human Aortic Smooth Muscle Cells

作　　者：郑辉[1] 刘慧娟[2] 葛焕琦[1] 张春风[1] 胡睿[1] 李雪粉[1] 

机构地区：[1]泰达国际心血管病医院内分泌科 [2]天津国际生物医药联合研究院,天津市300457

出　　处：《中国动脉硬化杂志》2016年第2期141-144,共4页Chinese Journal of Arteriosclerosis

基　　金：天津市滨海新区卫生局科技项目(2013BWK2006)

摘　　要：目的研究网膜素对氧化应激作用下的人主动脉平滑肌细胞胶原Ⅰ、Ⅳ表达的影响。方法将人主动脉平滑肌细胞系培养,细胞密度到达90%以上后,随机分为5组:对照组、氧化应激组、网膜素组、细胞外调节蛋白激酶(ERK)抑制剂组和丝裂原活化蛋白激酶(p38MAPK)抑制剂组。其中,对照组不增加任何处理,氧化应激组加入叔丁基氢过氧化物(t-BHP,87.1μmol/L),网膜素组(87.1μmol/L t-BHP+600μg/L网膜素)、ERK抑制剂组、p38MAPK抑制剂组为在网膜素组基础上分别加入ERK抑制剂(PD98059,60μmol/L)和p38MAPK抑制剂(SB203580,100μmol/L),应用Western blot法检测细胞中胶原Ⅰ、Ⅳ表达量。结果应用MTT法,筛选t-BHP最佳作用浓度为87.1μmol/L;与对照组相比,氧化应激组人动脉平滑肌细胞内Ⅰ、Ⅳ型胶原表达显著下降(P<0.01);与氧化应激组比较,网膜素组Ⅰ、Ⅳ型胶原表达显著升高(P<0.01);与网膜素组比较,ERK抑制剂组和p38MAPK抑制剂组Ⅰ、Ⅳ型胶原表达均显著下降(P<0.05)。结论网膜素能改善氧化应激对人动脉平滑肌细胞Ⅰ、Ⅳ型胶原表达抑制的作用,可能通过此机制促进动脉粥样硬化斑块的稳定,ERK/p38MAPK途径可能参与网膜素的信号传导。Aim To study the influence of omentin on the expression of collagen Ⅰ/Ⅳin the human aortic smooth muscle cells subjected to oxidative stress. Methods Human aortic smooth muscle cells were cultured in vitro.When cell density reached above 90%,cells were randomly divided into 5 groups： control group,oxidative stress group,omentin group,ERK（ extracellular regulated protein kinases） inhibitor group and p38MAPK（ p38 mitogen-activated protein kinases） inhibitor group. Among them,the control group did not receive any treatment,oxidative stress group were treated with tert-butyl hydroperoxide（ t-BHP,87. 1 μmol / L） to simulate the state of oxidative stress; omentin group were treated,except for 87. 1 μmol / L t-BHP,with 600 μg / L omentin; except for t-BHP（ 87. 1 μmol / L） and omentin（ 600 μg / L）,ERK inhibitors（ PD98059,60 μmol / L） and p38 MAPK inhibitors（ SB203580,100 μmol / L） were used in ERK inhibitor group and p38 MAPK inhibitor group respectively. The expression of collagen Ⅰ / Ⅳ were detected by Western blot hybridization. Results MMT method was used to screen the optimal concentration and the best action time of t-BHP.（ 1） The optimal concentration was 87. 1 μmol/L;（ 2） Compared with the control group,the expression of collagen Ⅰ/Ⅳin human aortic smooth muscle cells in oxidative stress group decreased significantly（ P〈0. 01）;（ 3） Compared with the oxidative stress group,the expression of type Ⅰ / Ⅳ collagen in human aortic smooth muscle cells increased significantly in omentin group（ P〈0. 01）;（ 4） Compared with the omentin group,the expression of collagen Ⅰ / Ⅳin ERK inhibitor group and p38 MAPK inhibitor group were decreased significantly（ P〈0. 05）. Conclusion Omentin could improve oxidative stress-induced down-regulation of Ⅰ / Ⅳcollagen in the human aortic smooth muscle cells,and probably promote the stabilization of atherosclerotic plaque through the mechanism. ERK / p38 MAPK signaling pathway may be involved in

关 键 词：网膜素 人主动脉平滑肌细胞 氧化应激 Ⅰ型胶原 Ⅳ型胶原 

分 类 号：R96[医药卫生—药理学]