胰岛素对大鼠平滑肌细胞表达MMP-9、TIMP-1及内皮细胞表达sES的影响  

Effect of Insulin on the Expression of MMP9 and TIMP-1 Released from Smooth Muscle Cells and E-selectin Released from Endothelial Cells in SD Rats

作　　者：葛晨亮[1] 刘厂辉[1] 唐振旺[1] 吴洁[1] 苏华[1] 王刚[1] 占凡[1] 谢楠[1] 陈仲[1] 

机构地区：[1]南华大学附属第一医院心血管内科,湖南省衡阳市421001

出　　处：《中国动脉硬化杂志》2016年第2期145-149,共5页Chinese Journal of Arteriosclerosis

基　　金：湖南省卫生厅科研基金(B2012-049)

摘　　要：目的探讨胰岛素对SD大鼠平滑肌细胞表达基质金属蛋白酶9(MMP-9)、组织抑制因子1(TIMP-1)以及内皮细胞表达可溶性E-选择素(s ES)的影响。方法原代培养SD大鼠颈内动脉平滑肌细胞及主动脉内皮细胞,用不同剂量的胰岛素作用后,以酶联免疫吸附实验(ELISA)分别测定上清液MMP-9、TIMP-1及s ES的水平。结果与对照组(无胰岛素)相比,胰岛素组MMP-9及s ES水平显著增高,TIMP-1则显著降低(P<0.05)。经Pearson相关分析显示,胰岛素浓度与MMP-9(r=0.815,P<0.001)及s ES(r=0.841,P<0.001)呈正相关,与组织抑制因子1(r=-0.740,P<0.001)呈负相关。结论胰岛素促进SD大鼠平滑肌细胞MMP-9及内皮细胞s ES表达,抑制平滑肌细胞TIMP-1表达,并呈剂量依赖性。Aim To investigate the effect of insulin on expression of MMP-9,TIMP-1 and s ES. Methods Primary cultured smooth muscle cells from the internal carotid artery and endothelial cells from the aorta of SD rats treated with different doses of insulin,MMP-9,TIMP-1 and s ES released by cells into medium were quantified by ELISA. Results MMP-9 and SES levels were higher in insulin group compared with control group,while TIMP-1 was less than control group（ P〈0. 05）. Significant positive correlation was observed between insulin and MMP-9（ r = 0. 815,P〈0. 001） as well as s ES（ r = 0. 841,P〈0. 001）,negative correlation was observed between insulin and TIMP-1（ r =- 0. 740,P〈0. 001）. Conclusion Insulin augmented the secretion of SES and MMP-9,attenuated the secretion of TIMP-1 in dependence on the concentration.

关 键 词：胰岛素 基质金属蛋白酶9 组织抑制因子1 E-选择素 

分 类 号：R543.3[医药卫生—心血管疾病]