Neuroprotection of Chrysanthemum indicum Linne against cerebral ischemia/reperfusion injury by anti-inflammatory effect in gerbils  

作　　者：Ki-YeonYoo In Hye Kim Jeong-Hwi Cho li Hyeon Ahn Joon Ha Park Jae-Chul Lee Hyun-Jin Tae Dae Won Kim Jong-Dai Kim Seongkweon Hong Moo-Ho Won il Jun Kang 

机构地区：[1]Department of Oral Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University, Gangneung,Republic of Korea [2]Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea [3]Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, Republic of Korea [4]Department of Biochemistry and Molecular Biology, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju NationalUniversity, Gangneung, Republic of Korea [5]Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Republic of Korea [6]Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, Republic of Korea [7]Department of Food Science and Nutrition, Hallym University, Chuncheon, Republic of Korea

出　　处：《Neural Regeneration Research》2016年第2期270-277,共8页中国神经再生研究（英文版）

基　　金：supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Science,ICT and Future Planning(NRF-2013R1A2A2A01068190);Hallym University Specialization Fund(HRF-S-13)

摘　　要：In this study, we tried to verify the neuroprotective effect of Chrysanthemum indicum Linne（CIL） extract, which has been used as a botanical drug in East Asia, against ischemic damage and to explore the underlying mechanism involving the anti-inflammatory approach. A gerbil was given CIL extract for 7 consecutive days followed by bilateral carotid artery occlusion to make a cerebral ischemia/reperfusion model. Then, we found that CIL extracts protected pyramidal neurons in the hippocampal CA1 region（CA1） from ischemic damage using neuronal nucleus immunohistochemistry and Fluoro-Jade B histofluorescence. Accordingly, interleukin-13 immunoreactivities in the CA1 pyramidal neurons of CIL-pretreated animals were maintained or increased after cerebral ischemia/reperfusion. These findings indicate that the pre-treatment of CIL can attenuate neuronal damage/death in the brain after cerebral ischemia/reperfusion via an anti-inflammatory approach.In this study, we tried to verify the neuroprotective effect of Chrysanthemum indicum Linne（CIL） extract, which has been used as a botanical drug in East Asia, against ischemic damage and to explore the underlying mechanism involving the anti-inflammatory approach. A gerbil was given CIL extract for 7 consecutive days followed by bilateral carotid artery occlusion to make a cerebral ischemia/reperfusion model. Then, we found that CIL extracts protected pyramidal neurons in the hippocampal CA1 region（CA1） from ischemic damage using neuronal nucleus immunohistochemistry and Fluoro-Jade B histofluorescence. Accordingly, interleukin-13 immunoreactivities in the CA1 pyramidal neurons of CIL-pretreated animals were maintained or increased after cerebral ischemia/reperfusion. These findings indicate that the pre-treatment of CIL can attenuate neuronal damage/death in the brain after cerebral ischemia/reperfusion via an anti-inflammatory approach.

关 键 词：nerve regeneration transient cerebral ischemia delayed neuronal death pyramidal neurons inflammatory cytokines neural regeneration 

分 类 号：R285.5[医药卫生—中药学]