心痛泰对动脉硬化兔主动脉NF-κBp65、TNF-α、IL-10的影响  

Study on Effects of Xintongtai on NF-κB p65, TNF-α and IL-10 in Aortas of Rabbits with Atherosclerosis

作　　者：易琼[1] 郭志华[2] 李雅[2] 任欣[2] 申思[2] 蒋文良 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《中医药导报》2016年第6期32-36,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省中医药科研项目(编号:201516)

摘　　要：目的:观察心痛泰对动脉粥样硬化(AS)兔的血脂及主动脉组织NF-κB-p65、TNF-α和IL-10的影响。方法:120只清洁级日本大耳白兔随机分为6组,空白组、模型组、心痛泰高剂量、中剂量及低剂量组、瑞舒伐他汀钙组,采用高脂喂养加免疫损伤造模,造模同时予以相应药物灌胃。60 d后,采血测定血脂,取胸主动脉用免疫组化法测定TNF-α、IL-10,用Rt-PCR法测定NF-κB-p65的m RNA表达,Western Blot法测定NF-κB-p65的蛋白表达。结果:心痛泰各组和瑞舒伐他汀钙组降低血脂的差异有统计学意义(P<0.01,P<0.05)。与模型组比较,心痛泰中、高剂量组和瑞氟伐他汀钙组动脉组织中的NF-κB-p65、TNF-α、IL-10的变化的差异有统计学意义(P<0.01)。结论:心痛泰能减轻AS炎症反应,作用机制可能是通过降低血脂、减少NF-κB-p65和TNF-α的表达,提高抗炎因子IL-10,从而减轻血管炎症,达到治疗AS的目的。Objective： To study the effects of Xintongtai（心痛泰, XTT） on levels of serum lipid and aortic NF-κB p65, TNF-α and IL-10 in rabbits with atherosclerosis. Methods： A total of 120 clean grade healthy Japanese rabbits were randomly divided into control group, model group, low-dose XTT group, middle-dose XTT group, high-dose XTT group and Rosuvastatin group. All rabbits were sacrificed by high-fat diet and immunologic injury. The drug intervention groups were treated with the corresponding drugs for 60 days, then serum lipid, tumor necrosis factor-α（TNF-α）, interleukin-6（IL-10） were tested by Immunohistochemistry. The activity expression of NF-κB p65 in thoracic aortas was tested by Western Blot, and NF-κB-p65-m RNA was tested by Real-time quantitative PCR（Rt-PCR）. Results： Compared with model group, the XTT and Rosuvastatin group could decrease the content of blood lipid（P〈0.01, P〈0.05）. Variation of NF-κB-p65, IL-10 and TNF-α in the middle-dose XTT, high-dose XTT and Rosuvastatin groups were significant than model group（P〈0.01）. Conclusions： The XTT can reduce inflammatory response of atherosclerosis, and its mechanism probably related to lower serum lipid, reduce the expression of NF-κB-p65, TNF-α, and promote the expression of IL-10.

关 键 词：动脉粥样硬化 心痛泰 TNF-α IL-10 NF-κB-p65 

分 类 号：R285.5[医药卫生—中药学]