头孢他啶对铜绿假单胞菌生物膜形成的影响与机制的探讨  

Effect of ceftazidime of formation of biofilm of Pseudomonas aeruginosa and mechanism

作　　者：李琬琛[1] 宋林[2] 李立艳[1] 孙续国[3] 魏爱琳 魏殿军[1] 

机构地区：[1]天津医科大学第二医院检验科,天津300211 [2]天津市中医药研究院检验科,天津300120 [3]天津医科大学医学检验学院,天津300203 [4]济南市大学药学院,山东济南200012

出　　处：《中华医院感染学杂志》2016年第6期1212-1215,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(30973157)

摘　　要：目的探讨头孢他啶在铜绿假单胞菌(PAE)生物膜形成过程中的抑制、清除作用与机制,更好的指导临床用药。方法通过96孔板结晶紫染色方法,定量分析头孢他啶对PAE标准菌株生物膜的抑制、清除作用,通过real time-PCR方法对生物膜形成相关基因的表达进行相对定量分析,探讨头孢他啶对PAE生物膜的作用机制。结果未形成成熟生物膜时,头孢他啶对PAE生长的抑制效果较好,而在有成熟生物膜形成时,头孢他啶不能有效抑制PAE的生长;头孢他啶与PAE生物膜形成相关基因的增多表达相关。结论头孢他啶能够促进PAE生物膜形成相关基因的表达,临床对慢性PAE感染患者的治疗应避免使用头孢他啶。OBJECTIVE To observe the effect of ceftazidime on inhibition of formation and clearance of Pseudomonas aeruginosa biofilm and analyze the mechanisms so as to provide guidance for clinical use of antibiotics.METHODS The 96 well plate crystal violet staining method was used to quantitatively analyze the effect of ceftazidime on inhibition and clearance of the P.aeruginosabiofilm,and the real-time PCR method was employed to perform the relative quantitative analysis of the expression of biofilm formation-related genes so as to observe the effect of ceftazidime on the formation of P.aeruginosa biofilm.RESULTS Ceftazidime had better effect on inhibition of growth of P.aeruginosa when the mature biofilms were not formed,however,ceftazidime could not effectively inhibit the growth of P.aeruginosa when the mature biofilms were formed.Ceftazidime was associated with the increased expression of the biofilm formation-related genes of P.aeruginosa.CONCLUSION Ceftazidime can promote the expression of the biofilm formation-related genes of P.aeruginosa.It is necessary for the hospital to avoid the use of ceftazidime for the treatment of the patients with chronic P.aeruginosainfection.

关 键 词：铜绿假单胞菌 生物膜 头孢他啶 

分 类 号：R378.99[医药卫生—病原生物学]