组蛋白去甲基化酶JMJD2和雌激素受体相关受体α在绝经后骨质疏松症的表达  

Expression of jumonji domain-containing histone demethylase 2 and estrogen-related receptor alpha in postmenopausal osteoporosis

作　　者：唐宏宇[1,2] 董路珏[3] 霍少川[3] 郭承[3] 周驰[1,2] 陈建发[1] 刘勇[3] 王海彬[1,2] 

机构地区：[1]广州中医药大学第一附属医院骨科中心,广东省广州市510405 [2]广州中医药大学国家重点骨科实验室,广东省广州市510405 [3]广州中医药大学,广东省广州市510405

出　　处：《中国组织工程研究》2016年第2期167-172,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81373655);广东省省级科技计划项目(2013B021800226)~~

摘　　要：背景:研究发现组蛋白去甲基化酶具有促进成骨细胞分化的作用;雌激素相关受体α可促进成骨细胞分化,增加骨形成。然而绝经后骨质疏松症组蛋白去甲基化酶表达以及与雌激素受体相关受体α的相关性未见报道。目的:观察绝经后骨质疏松症患者组蛋白去甲基化酶2家族的变化及其对组蛋白甲基化(H3K9me3、H3K36me3)水平的影响,以及与雌激素受体相关受体α的相关性。方法:选择年龄50-70岁因髋关节骨性关节炎行关节置换的绝经后患者,通过检测腰椎骨密度,收集10例绝经后骨质疏松症患者(实验组)和10例非骨质疏松症患者(对照组),术中提取股骨头松质骨标本,免疫组织化学、蛋白质印迹法检测骨组织中组蛋白去甲基化酶(JMJD2A、JMJD2B)、组蛋白甲基化(H3K9me3、H3K36me3)以及雌激素受体相关受体α的表达。结果与结论:绝经后妇女组蛋白去甲基化酶2A、组蛋白去甲基化酶2B、雌激素受体相关受体α表达为弱阳性到阳性不等,骨质疏松症患者组表达水平低于非骨质疏松症患者组,组间差异有显著性意义(P<0.05);骨质疏松症患者组H3K9me3、H3K36me3表达水平高于非骨质疏松症患者组,组间差异有显著性意义(P<0.05)。结果说明,绝经后骨质疏松症患者组蛋白呈高甲基化状态,组蛋白去甲基化酶2A、组蛋白去甲基化酶2B低表达,雌激素受体相关受体α水平与组蛋白去甲基化酶相一致;组蛋白去甲基化酶可能为骨质疏松的一种拮抗酶。BACKGROUND： Jumonji domain-containing histone demethylase（JMJD） can promote osteoblast differentiation, and estrogen-related receptor alpha（ERRα） can promote osteoblast differentiation and increase bone formation. However, little is reported on the association between postmenopausal osteoporosis and JMJD and ERRα. OBJECTIVE： To study the changes in the JMJD2 family expression in patients with postmenopausal osteoporosis. METHODS： Postmenopausal patients with osteoarthritis of the hip scheduled for total hip arthroplasty, aged 50-70 years, were enrolled, including 10 postmenopausal osteoporosis patients（experimental group） and 10 patients with no postmenopausal osteoporosis（control group）. During the arthroplasty, the cancellous bone specimens from the femoral head were collected. Then, immunohistochemistry and western blot assay were used to detect expression of histone demethylase（JMJD2A, JMJD2B）, histone methylation（H3K9me3, H3K36me3） and ERRα. RESULTS AND CONCLUSION： In the experimental group, the expressions of JMJD2 A, JMJD2 B and ERRα were from weakly positive to positive; these expressions were significantly lower in the experimental group than the control group（P〈0.05）. The expressions of H3K9me3 and H3K36me3 were significantly higher in the experimental group than the control group（P〈0.05）. These findings indicate that the expression of JMJD2 A and JMJD2 B is consistent with the expression of ERRα in the patients with postmenopausal osteoporosis, and JMJD is likely to serve as an antagonistic enzyme of osteoporosis.

关 键 词：骨质疏松 遗传学 组蛋白类 甲基化 受体 雌激素 组织工程 组织构建 骨组织工程 绝经后骨质疏松症 表观遗传学 组蛋白去甲基化酶JMJD2 免疫组织化学 蛋白质印迹 雌激素受体相关受体α 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]