上、下坡跑台训练膝骨关节炎模型大鼠关节软骨及炎症因子的变化  

Effect of uphill or downhill running training on articular cartilage degeneration and inflammatory responses of knee osteoarthritis rats

作　　者：王文胜[1] 陈伟[1] 沈堡垒 许波[1] 张俐[1] 江征[1] 

机构地区：[1]福建中医药大学,福建省福州市350122

出　　处：《中国组织工程研究》2016年第2期197-201,共5页Chinese Journal of Tissue Engineering Research

基　　金：福建省教育厅A类科技项目(JA12167)~~

摘　　要：背景:不同的训练方式对原发性膝骨关节炎模型的建立都会产生不同的影响。目的:对比观察上坡和下坡跑台训练在诱导大鼠膝骨关节炎模型过程中对膝关节软骨变性及炎症反应的作用。方法:SD雌性大鼠108只,随机等分为6组:空白组、上坡跑步、下坡跑步组、去卵巢模型组、模型+上坡跑步组和模型+下坡跑步组。空白组大鼠常规饲养不做任何处理;上坡跑步组大鼠常规饲养并进行+15°跑台训练;下坡跑步组大鼠常规饲养并进行-15°跑台训练;模型组大鼠行双侧卵巢切除后常规饲养;模型+上坡跑步组大鼠去卵巢后进行+15°跑台训练;模型+下坡跑步组去卵巢后进行-15°跑台训练。跑台训练的频率均为28 m/min,60 min/d,6 d/周。结果与结论:造模4周时,模型+上坡跑步组大鼠Mankin评分明显较另5组增高,已达软骨损伤标准,尿液中Ⅱ型胶原羧基端肽浓度已过峰值期;6周时模型+下坡跑步组大鼠Mankin评分达到了软骨损伤的标准,较模型+上坡跑步组低,模型+上坡跑步组和模型+下坡跑步组大鼠肿瘤坏死因子α、白细胞介素1β质量浓度均较其余4组增高;8周时模型+上坡跑步组和模型+下坡跑步组大鼠Mankin评分及关节液肿瘤坏死因子α、白细胞介素1β质量浓度进一步增加,其中模型+上坡跑步组更为严重。结果提示去势后联合疲劳性跑台训练,上坡和下坡训练均可造成大鼠关节软骨的损伤及关节局部炎症反应,上坡训练与下坡训练相比,软骨损伤出现较早,效率更高。BACKGROUND： Different training methods have different effects on primary knee osteoarthritis. OBJECTIVE： To compare the effects of uphill or downhill running on articular cartilage degeneration and inflammatory responses of knee osteoarthritis rats.METHODS： 108 female Sprague-Dawley rats were randomly divided into six groups： normal control group, ovariectomized group, uphill running group（run up）, downhill running group（run down）, uphill running model group（model up）, downhill running model group（model down）. The rats in the normal control group were raised routinely with no treatment; rats in the ovariectomized group were ovarientomized and raised in routine way; rats in the run up group and run down group were subjected to uphill running training on the slop ＋15° or downhill running training on the slop-15°; rats in the model up group and model down group were subjected to uphill running training on the slop ＋15°or downhill running training on the slop-15° after ovarientomized. Training programs were as follows： 28 m/min, 60 minutes per day, 6 days per week. RESULTS AND CONCLUSION： Compared with the other five groups, in the model up group, the Mankin＇s score was significantly increased, indicating that articular cartilage degeneration occurred, and the peak of carboxy-terminal telopeptide of type Ⅱ collagen contained in the urine had passed, at 4 weeks after modeling. At 6 weeks, the Mankin＇s score of the model down group was increased to reach the degeneration standard, but it was still lower than that of the model up group; the expression levels of tumor necrosis factor-α and interleukin-1β in the synovial fluid were significantly higher in the model up and model down groups than the other four groups. At 8 weeks, the Mankin＇s score and the expression levels of tumor necrosis factor-α and interleukin-1β were further increased in the model up and model down groups, especially in the model up group. These results indicate that treadmill running exercise after ov

关 键 词：骨关节炎 膝 炎症 组织工程 组织构建 软骨组织工程 膝骨关节炎 动物模型 跑台训练 去卵巢 软骨 关节液 尿液 

分 类 号：R318[医药卫生—生物医学工程]