支架涂层材料对血管平滑肌的影响  

Effect of Stent Coating Material on Vascular Smooth Muscle Cells

作　　者：赵鸿彦[1] 石蓓[1] 赵然尊[1] 张文航 

机构地区：[1]遵义医学院附属医院心血管内科,贵州遵义563003

出　　处：《医学临床研究》2016年第2期220-222,共3页Journal of Clinical Research

基　　金：贵州省科学技术基金项目编号：黔科合J字LKZ[2012]26号

摘　　要：【目的】 探讨支架涂层材料对血管平滑肌的影响。【方法】 以SD大鼠主动脉血管平滑肌细胞为载体，采用MTT试验检测苯乙烯丁烯共聚物及多聚乳酸涂层材料对血管平滑肌细胞的增殖能力的影响，流式细胞仪检测细胞周期。【结果】 随着培养天数增加，苯乙烯丁烯共聚物组和多聚乳酸组平滑肌细胞数量都呈递增趋势；相同时间内苯乙烯丁烯共聚物组和多聚乳酸组细胞数量显著低于对照组（P〈0.05），但苯乙烯丁烯共聚物组与多聚乳酸组差异无显著性（P＞0.05）；与对照组相比，苯乙烯丁烯共聚物及多聚乳酸组细胞周期均有不同程度变化，主要表现为G0/G1期细胞数显著高于对照组，S期、G2/M期细胞数显著低于对照组，差异具有统计学意义（P〈0.05），苯乙烯丁烯共聚物组与多聚乳酸组比较差异无统计学意义（P〉0.05）。【结论】苯乙烯丁烯共聚物及多聚乳酸可能通过抑制细胞周期中的第1个调控点（G1→S限制点）抑制主动脉血管平滑肌细胞增殖，两种涂层材料之间无明显差异。【Objective】To investigate the effect of stent coating material on vascular smooth muscle cells. 【Methods】Vascular smooth muscle cells of SD rats were used for testing. The effects of butylene styrene copolymer and polylactic acid coating material on production ability of vascular smooth muscle cells was observed. Flow cemetery method was used to determine cell cycle. 【Results】The number of vascular smooth muscle cells in both the butylene styrene copolymer and polylactic acid group increased with the culture time. The number of vascular smooth muscle cells in both the polylactic acid groups and butylene styrene copolymer group was significantly lower than in the control group （P〈0.05）. Compared with the control group, butylene styrene copolymer and polylactic acid cell cycle changes were in varying degrees, mainly for the G0/G1 phase cells increased, S phase, G2/M phase cells decreased（P〈0.05）. There was no significant difference between the butylene copolymer of styrene and polylactic acid group （P〉0.05）. 【Conclusion】Butylene styrene copolymer and polylactic acid, mainly through the regulating point （G1→S turning point）, inhibited production of vascular smooth muscle cell.

关 键 词：支架 涂料 肌 平滑 血管 

分 类 号：R685.6[医药卫生—骨科学]