秋水仙素和^(60)Co-γ射线对无芒隐子草种子萌发的影响  

Effect of colchicines and ^(60)Co-γ-rays on germination of Cleistogenes songorica seeds

作　　者：张代玉[1] 吴凡[1] 张吉宇[1] 王彦荣[1] 张岩[1] 罗栋[1] 

机构地区：[1]草地农业生态系统国家重点实验室兰州大学草地农业科技学院,甘肃兰州730020

出　　处：《草业科学》2016年第3期424-430,共7页Pratacultural Science

基　　金：科技部"973"课题(2014CB138704);国家自然科学基金(31572453)

摘　　要：通过不同剂量秋水仙素和^(60) Co-γ射线处理,系统分析了无芒隐子草(Cleistogenes songorica)种子的发芽率、发芽指数及幼苗生长。结果表明,秋水仙素处理对无芒隐子草种子的发芽率和发芽指数有一定程度的抑制作用,经0.1%浓度处理48h的种子,其发芽率和发芽指数分别为对照组的54.3%和43.3%,分别是41%和0.55;不同剂量^(60) Co-γ射线对无芒隐子草种子的发芽率、发芽指数及幼苗生长产生不同的影响。低剂量(50~200Gy)提高种子发芽率和发芽指数,高剂量(400~2 000Gy)抑制种子发芽率、发芽指数及幼苗生长。其中,^(60)0Gy辐射处理的发芽率为48.5%。因此,0.1%浓度处理48h和^(60)0Gy可分别作为秋水仙素和^(60) Co-γ射线处理的半致死剂量。本研究初步确定的半致死剂量,可为进一步研究无芒隐子草种质资源创新提供理论依据。This experiment systematically analyzed germination percentage,germination index and seedlings growth of Cleistogenes songorica with different doses of colchicine＇s and ^（60）Co-γ-rays treatments.The results showed that colchicine had certain degree of inhibition on seed germination of C.songorica.With the treatment of 0.1% colchicine solution for 48 h,the germination percentage and germination index were 54.3%and 43.3%of the control which were 41%and 0.55,respectively.However,the effects of ^（60）Co-γ-rays on germination percentage,germination index and the growth of seedlings varied with does.Low doses（50~200Gy）increased germination percentage and germination index whereas high doses（400~2 000Gy）inhibited the germination percentage,germination index and seedling growth.The germination percentage was 48.5% under the treatment of^（60）0 Gy.The present study preliminary determined the semi-lethal dose treatment was 0.1%colchicine solution for 48 hand ^（60）0Gy ^（60）Co-γ-rays,respectively,which provided a theoretical basis for the innovation of germplasm resources in C.songorica.

关 键 词：无芒隐子草 秋水仙素 60 CO-Γ射线 发芽 

分 类 号：S543.9[农业科学—作物学]