超顺磁性Fe_3O_4@SiO_2空心亚微球的制备及其在质粒DNA分离纯化中的应用  

Fabrication of superparamagnetic Fe_3O_4@SiO_2 hollow sub-microspheres and their application in plasmid DNA purification

作　　者：刘曼丽[1] 张玉玉[1] 刘春巧[1] 刘长霞[1] 

机构地区：[1]北京化工大学生命科学与技术学院,北京100029

出　　处：《北京化工大学学报（自然科学版）》2016年第2期58-62,共5页Journal of Beijing University of Chemical Technology(Natural Science Edition)

基　　金：国家自然基金(21406012);厦门市对台科技合作项目(3502Z20151226);中央高校基本科研业务费(ZY1417/YS1407)

摘　　要：采用溶剂热法合成超顺磁性空心亚微球,然后通过正硅酸乙酯水解-聚合反应,在亚微球表面包覆SiO_2,形成核壳结构Fe_3O_4@SiO_2空心亚微球。以该Fe_3O_4@SiO_2亚微球为分离介质,实现了大肠杆菌(E.coli)质粒DNA的高效、快速分离。Monodisperse superparamagnetic Fe3O4 sub-microspheres with a hollow structure have been fabricated via a facile solvothermal route. Fe3O4 @ SiO2 was subsequently prepared by treating sub-microspheres with tetraethoxysilane in a hydrolysis-polymerization process and the product used for E. coli plasmid DNA purification. The experiments demonstrated that Fe3O4@ SiO2 can be used for fast and efficient purification of plasmid DNA.

关 键 词：Fe3O4@SiO2 超顺磁性空心亚微球 质粒DNA 纯化 

分 类 号：Q781[生物学—分子生物学]