非小细胞肺癌中D2-40、podoplanin的表达与淋巴结微转移的关系  

The relationship between the expression of D2-40,podoplanin in non small cell lung cancer with lymph node micrometastasis

作　　者：王立红[1] 付秀华[1] 高志芳[1] 高俊珍[1] 王美玲 顾岩[1] 贺岚[1] 

机构地区：[1]内蒙古医科大学附属医院呼吸内科,内蒙古呼和浩特010050

出　　处：《中国医刊》2016年第3期47-50,共4页Chinese Journal of Medicine

基　　金：内蒙古科技厅自然科学基金(2012MS1183)

摘　　要：目的探讨单克隆抗体D2-40、肾小球足突细胞膜黏蛋白(podoplanin)在非小细胞肺癌(non small cell lung cancer,NSCLC)淋巴微转移中的临床意义。方法随机收集本院100例NSCLC手术患者,其中术后病理证实34例出现淋巴结转移,66例患者未出现淋巴结转移,随访后把66例患者分为复发组和无复发组,复发组有23例,无复发组有43例。30例肺部良性疾病患者作为对照组。采用免疫组织化学方法检测实验组癌组织、癌旁组织及对照组患者病变组织中D2-40及podoplanin的表达。结果D2-40和podoplanin在NSCLC复发组、无复发组及对照组中微淋巴管密度(lymphatic microvessel density,LMVD)计数分别为(12.67±2.06)个、(9.53±2.04)个、(4.83±2.02)个和(18.12±2.28)个、(13.48±2.16)个、(9.93±2.10)个,两者在复发组中LMVD计数均显著高于其他两组(P<0.01);D2-40和podoplanin在Ⅰ、Ⅱ、Ⅲ期NSCLC患者中的LMVD分别为(8.43±1.89)个、(9.36±1.92)个、(11.58±1.95)个和(12.28±2.05)个、(14.13±2.08)个、(17.49±2.13)个,两个因子在NSCLC不同分期中的表达差异均有显著性(P<0.05)。结论 D2-40、podoplanin的表达均与NSCLC患者淋巴转移及临床分期密切相关,两者可以特异性识别NSCLC组织中淋巴管内皮细胞,其标记的淋巴管主要位于癌周组织。联合检测可提高NSCLC淋巴转移的诊断率,更准确地进行TNM分期,为NSCLC患者治疗方案的选择及预后评估提供可靠证据。Objective To investigate the clinical significance of testing the monoclonal antibody（D2-40）,glomerular foot process membrane mucins（podoplanin） in micrometastases of non-small cell lung cancer（non small cell lung cancer,NSCLC）.Method Randomly collected 100 cases of clinical patients with NSCLC in our hospital,including 34 patients with lymph node metastases,66 cases of patients with no lymph node metastasis occurred,after follow-up of 66 patients were divided into two groups,recurrence group and non recurrent group.There were 23 cases of recurrence group and there were 43 cases of non recurrence group.30 patients with lung benign disease as the control group.Selected the immunohistochemical method to detect D2-40 and podoplanin expression in cancer tissues and adjacent tissues of the experimental group and lesions of control group.Result The counts of LMVD of D2-40 and podoplanin in relapse group and no recurrence and pulmonary benign lesions group were respectively：（12.67±2.06）,（9.53±2.04）,（4.83±2.02） and（18.12±2.28）,（13.48±2.16）,（9.93±2.10）.The counts of LMVD both of D2-40 and podoplanin in recurrence group were significantly higher than the other two groups（P〈0.01）.The counts of LMVD of D2-40 and podoplanin in clinical staging ofⅠ,Ⅱ,Ⅲ respectively：（8.43±1.89）,（9.36±1.92）,（11.58±1.95） and（12.28±2.05）,（14.13±2.08）,（17.49±2.13）.The expression of the two factors in the different staging of NSCLC were significantly different（P〈0.05）.Conclusion The expression of the D2-40,podoplanin was closely related with lymph node metastasis and clinical stage.The two factors can specific recognized lymphatic endothelial cell in the organization of NSCLCT.The markers of lymphatic in cancerous peripheral tissue.The combined detection of the factors can improve the diagnosis rate of lymph micrometastasis and more accurate TNM staging in NSCLC.To provide reliable evidence for the selection and evaluation of prognosis of patients with the

关 键 词：非小细胞肺癌 微转移 单克隆抗体 肾小球足突细胞膜黏蛋白 免疫组化 

分 类 号：R563.9[医药卫生—呼吸系统]