芪雪浸膏剂质量标准研究  

Study on the quality standards of Qixue Concrete

作　　者：陆超[1,2] 吴磊 钱芳 刘志辉 

机构地区：[1]南京中医药大学附属医院药学部,南京210029 [2]南京中医药大学研究生院,南京210046

出　　处：《中国中医药现代远程教育》2016年第4期135-138,共4页Chinese Medicine Modern Distance Education of China

摘　　要：目的研究并建立芪雪浸膏剂的质量控制标准。方法采用薄层色谱法对芪雪浸膏剂中的黄芪、当归、虎杖、大黄、进行定性鉴别;采用HPLC-ELSD法研究并建立芪雪浸膏剂中黄芪甲苷的含量测定方法。结果处方中4味中药均能在薄层色谱中检出,分离效果好,斑点清晰,阴性未见干扰。黄芪甲苷进样量在2.5~25μg范围内呈良好线性关系（r=0.999 2）,平均回收率96.62%,RSD为1.42%（n=6）。结论建立的方法简便、可行、准确,可为芪雪浸膏剂质量标准的制定提供依据。Objective To establish the quality standards for Qixue concrete. Methods Astragali Radix, Angelicae Sinensis Radix,Polygoni Cuspdati Rhizoma Et Radix, Smilacis Glabrae Rhizoma, Rhei Radix Et Rhizoma, Centeliae Herba and Abelmoschi Corolia in Qixue concrete were qualitatively identified by TLC. The content of astragaloside Ⅳ was determined by HPLC-ELSD. Results Six herbs could be identified by TLC, and the spots were clear and well-separated. The linear range of Astragaloside Ⅳ was 2.5~25. Μg（r=0.999 2）, and the average recovery was 96.62 %, RSD=1.42%（n=6）. Conclusion The method is simple and accurate, and it can be used for the quality control of Qixue concrete.

关 键 词：芪雪浸膏剂 质量标准 薄层色谱 高效液相色谱 黄芪甲苷 

分 类 号：R286.0[医药卫生—中药学]