合成新型荧光增强型探针用于巯基蛋白质的检测  

Synthesis of new fluorescence-enhanced probe for detection of thiol proteins

作　　者：杨玲[1] 廖超强 曹杰[1] 张学俊[1] 

机构地区：[1]中北大学理学院,山西太原030051

出　　处：《现代化工》2016年第3期178-181,183,共5页Modern Chemical Industry

基　　金：国家自然科学基金资助项目(51272239);山西省自然科学基金资助项目(2011011022-4);山西省回国留学人员科研资助项目(2015-078)

摘　　要：基于光致电子转移原理(Photoinduced Electron Transfer,Pe T),建立了以5-(4-马来酰亚胺基苯基)-10,15,20-三(4-甲基苯基)卟啉(MPTMPP)为荧光增强型探针,对巯基蛋白质特异性进行检测。以吡咯、对硝基苯甲醛、对甲基苯甲醛等为主要原料合成了目标化合物MPTMPP,并通过核磁、IR光谱等对MPTMPP进行表征;探究了MPTMPP作为荧光探针对含有巯基的牛血清白蛋白(BSA)的特异性检测效果,该探针对BSA检测的线性范围为0~2.0×10^(-8)mol/L,线性方程为ΔF=68.588+36.524[BSA](10^(-8)mol/L),相关系数为0.996 9,检出限为0.037 nmol/L。5-（ 4-Maleimidophenyl）-10,15,20-tri-（ 4-methyphenyl） porphrin（ MPTMPP） is synthesized by using pyrrole,4-nitrobenzaldehyde and 4-methylbenzaldehyde as the main raw materials,which is characterized by NMR,FTIR,etc.. It is then used as a fluorescene-enhanced probe for specific detection of thiol-proteins based on the principle of photoinduced electron transfer（ PET）. The specific detection effect of BSA is performed. The linear range for detection of BSA is 0- 2. 0 × 10^-8mol / L with linear equation as ΔF = 68. 588 ＋ 36. 524[BSA ]（ 10^-8mol / L） and correlation coefficient as 0. 996 9. The detection limit for BSA is 0. 037 nmol / L.

关 键 词：卟啉 马来酰亚胺 荧光探针 荧光增强 牛血清白蛋白 

分 类 号：O656[理学—分析化学]