Hybridization between salt resistant and salt susceptible genotypes of mungbean(Vigna radiata L.Wilczek)and purity testing of the hybrids using SSRs markers  

作　　者：Sehrawat Nirmala Yadav Mukesh Bhat Kangila Venkataraman Sairam Raj Kumar Jaiwal Pawan Kumar 

机构地区：[1]Centre for Biotechnology,Maharshi Dayanand University,Rohtak 124001,India [2]Department of Biotechnology,Maharishi Markandeshwar University,Ambala 133207,India [3]National Bureau of Plant Genetic Resources,Pusa Campus,New Delhi 110012,India [4]Indian Society for Plant Physiology,G-3,NASC Complex,Pusa,New Delhi 110012,India

出　　处：《Journal of Integrative Agriculture》2016年第3期521-527,共7页农业科学学报（英文版）

摘　　要：Six cultivated and two wild genotypes of mungbean（Vigna radiata L. Wilczek） possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod set and significant variations in cross-compatibility of investigated genotypes. Genotypes PLM 380 and PLM 562 showed promising combining ability with all genotypes. Results revealed significant crossing compatibility between V. radiata and V. sublobata. The cross ability ranged from 1.99 to 5.12%（average 3.08%）. Molecular analysis confirmed the hybrids purity. All F_1 seeds were bold, green/shiny green and germinated between 3 to 5 days. Hybrid plants were normal, fertile and healthier over their respective progenitors. Uniform flowering and maturity of the hybrids showed absence of any defect or alteration in plant habit and life cycle of the hybrids. The hybrid plants showed increase in yield characteristics as no. of pods, pod length, 100 seeds weight, and yield per plant. Number of pods containing F_2 seeds ranged from 5–8 per cluster. The F_2 seeds were collected and stored for further research. Present study suggests that salt resistant wild relatives or cultivars of mungbean can be explored by breeding as a source of useful traits/genes providing salt tolerance. This may help in development of best mungbean variety for saline prone areas. Micro-satellite markers linked to the trait/genes may assist breeding by early selection of the genotypes compared to the phenotypic screening.Six cultivated and two wild genotypes of mungbean（Vigna radiata L. Wilczek） possessing variation for phenotypic and genotypic response for salt tolerance were hybridized. Hybridization results showed successful pod set and significant variations in cross-compatibility of investigated genotypes. Genotypes PLM 380 and PLM 562 showed promising combining ability with all genotypes. Results revealed significant crossing compatibility between V. radiata and V. sublobata. The cross ability ranged from 1.99 to 5.12%（average 3.08%）. Molecular analysis confirmed the hybrids purity. All F_1 seeds were bold, green/shiny green and germinated between 3 to 5 days. Hybrid plants were normal, fertile and healthier over their respective progenitors. Uniform flowering and maturity of the hybrids showed absence of any defect or alteration in plant habit and life cycle of the hybrids. The hybrid plants showed increase in yield characteristics as no. of pods, pod length, 100 seeds weight, and yield per plant. Number of pods containing F_2 seeds ranged from 5–8 per cluster. The F_2 seeds were collected and stored for further research. Present study suggests that salt resistant wild relatives or cultivars of mungbean can be explored by breeding as a source of useful traits/genes providing salt tolerance. This may help in development of best mungbean variety for saline prone areas. Micro-satellite markers linked to the trait/genes may assist breeding by early selection of the genotypes compared to the phenotypic screening.

关 键 词：mungbean salinity stress breeding hybrid purity improved characteristics 

分 类 号：S522[农业科学—作物学]