深海细菌Psychrobacter submarinus 1A01998的次级代谢产物研究  

Study on the secondary metabolites from the marine bacterium Psychrobacter submarinus 1A01998

作　　者：马小妮[1,2] 苗子 程轩轩[1] 杨全[1] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]国家海洋局第三海洋研究所海洋生物资源遗传重点实验室,福建厦门361005

出　　处：《广东药学院学报》2016年第1期25-28,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：国家海洋局第三海洋研究所基本科研业务费专项资金资助项目(2015028);海洋经济创新发展区域示范专项经费资助项目(GD2012-D01-001)

摘　　要：目的对来自深海沉积物中的海洋细菌Psychrobacter submarinus 1A01998的次级代谢产物进行研究。方法采用大孔吸附树脂、硅胶、ODS和Sephadex LH-20凝胶等柱层析色谱分离方法,对海洋细菌Psychrobacter submarinus 1A01998进行次级代谢产物的分离纯化,根据1H NMR、13C NMR和MS等波谱数据分析并结合相关文献对化学结构进行鉴定。结果从深海细菌Psychrobacter submarinus 1A01998的发酵粗提物中分离得到4个化合物,分别鉴定为环（L-脯氨酸-L-酪氨酸）（1）、环（D-脯氨酸-L-酪氨酸）（2）、3-吲哚甲醛（3）、2＇-O-甲氧基尿嘧啶核苷（4）。结论化合物1~4均为首次从该属中分离得到,其中化合物1、2为环二肽类化合物。Objective To study the secondary metabolites of Psychrobacter submarinus 1A01998,a deep-sea derived bacterium isolated from Pacific Ocean sediments. Methods Secondary metabolites were isolated and purified by column chromatography over macroporous resin,silica gel,ODS and Sephadex LH-20. The structures of secondary metabolites were identified by1 H NMR,13 C NMR,MS and reference data. Results Four secondary metabolites were obtained and identified as cylco（ L-Pro-L-Tyr）（ 1）,cylco（ D-Pro-L-Tyr）（ 2）,1H-indole-3-carboxaldehyde（ 3）,and 2＇-O-methyluridine（ 4）. Conclusion Compounds 1-4 were isolated from genus of Psychrobacter for the first time,and compounds 1 and 2 were cyclic dipeptides.

关 键 词：海洋细菌 PSYCHROBACTER submarinus 1A01998 次级代谢产物 

分 类 号：R915[医药卫生—微生物与生化药学]