生物诱导法所产沉香不同部位中3种色酮的含量测定  

Determination of 3 kinds of chromones in different parts of agarwood by biology induction

作　　者：张林杰[1] 吴泽青[1] 林励[1] 陈康[1] 李静[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《广东药学院学报》2016年第1期41-45,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家发改委高技术产业化项目(2011-56)

摘　　要：目的测定生物诱导法所得沉香不同部位色酮类成分的质量分数,评价生物诱导法刺激结香的效果。方法采用高效液相色谱法（HPLC法）测定生物诱导法诱导所得沉香不同部位中aquilarone B、aquilarone C及（5S,6R,7R,8S）-2-[2-（4-甲氧基-苯基）乙基]-5,6,7,8-四羟基-5,6,7,8-四氢色酮（CX16）的质量分数,色谱柱为waters symmetry shield C18柱（4.6 mm×250 mm,5μm）,流动相为乙腈-0.1%（体积分数）H3PO4,梯度洗脱,流速为1 m L/min,检测波长为234 nm,柱温为30℃,进样量为10μL。结果 Aquilarone B、aquilarone C、CX16分别在14.32~716、0.884~44.2、1.54~77μg/m L范围内与峰面积积分值呈良好线性关系。白木香木质部外侧黑色结香部分（B部分）中,B1样品的aquilarone B、aquilarone C质量分数最高,分别为3.411 8、1.021 6 mg/g,B4样品的CX16质量分数最高,为0.728 5 mg/g;而内侧棕色初结香部分（Z部分）所测得色酮质量分数显著低于B部分,其中Z1样品的aquilarone B、aquilarone C质量分数最高,分别为1.424 9、0.585 9 mg/g,Z4样品的CX16质量分数最高,为0.158 9 mg/g。结论生物诱导法诱导所得沉香不同部位3种色酮的质量分数有较大差异,木质部外侧黑色结香部分中3种色酮质量分数明显高于内侧棕色初结香部分,推测该法是由外而内诱导白木香木质部,逐步形成沉香药材。Objective To determine the chromones＇ contents in the agarwood generated by biology induction method and evaluate the effect of the new technology. Methods High performance liquid chromatography（ HPLC） was used for the determination of aquilarone B,aquilarone C and（ 5S,6R,7R,8S）-2-[2-（ 4-methoxy-phenyl）-ethyl]-5,6,7,8-tetrahydroxy-5,6,7,8-tetrahydro-chromones（ CX16） in different parts of the agarwood. Waters Symmetry Shield C18column（ 4. 6 mm × 250 mm,5 μm） was employed eluting gradiently with acetonitrile-0. 1% phosphoric acid water. The flow rate was 1 m L / min,and the detection wavelength was 234 nm. The column temperature was 30 ℃,and the injection volume was 10 μL. Results The linear ranges of aquilarone B,aquilarone C and CX16 were 14. 32- 716,0. 884-44. 2,and 1. 54- 77μg /m L,respectively. B1 sample contained the highest contents of aquilarone B（ 3. 411 8mg /g） and aquilarone C（ 1. 021 6 mg / g） among the knot incense part（ part B） located outside of Aquilaria sinensis xylem,and the highest content of CX16 is 0. 728 5 mg / g in B4 sample. The highest contents of aquilarone B（ 1. 424 9 mg / g） and aquilarone C（ 0. 585 9 mg / g） among the little knot incense part（ part Z） located inside of Aquilaria sinensis xylem was in Z1 sample,and the highest content of CX16 is 0.158 9 mg / g in Z4 sample among part Z. Conclusion The contents of 3 chromones varied in different parts of the agarwood induced by biological stimulation. They were significantly higher in part B than in part Z. The new method might affect the xylem with generating agarwood gradually from outside to inside.

关 键 词：生物诱导法 沉香 高效液相色谱法 色酮 

分 类 号：R284.1[医药卫生—中药学]