HPLC法测定印度黄檀中黄檀素等3种成分含量  

Quantitative determination of dalbergin and other two compounds in Dalbergia sissoo Roxb. by HPLC

作　　者：李然[1] 林励[1] 祁龙凯 杜海芳 李静[1] 蔡聪 郑来安 叶耀祥 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广东省湛江南药试验场,广东遂溪524338 [3]广东中檀实业有限公司,广东台山529200

出　　处：《广东药学院学报》2016年第1期51-54,共4页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的建立印度黄檀中黄檀素、黄檀素甲醚和4-甲氧基黄檀醌的质量分数测定方法。方法色谱柱为Waters Symmetry C18柱（4.6 mm×250 mm,5μm）,流动相为甲醇-0.2%（体积分数）H3PO4水溶液;梯度洗脱,流速为1.0 m L/min,柱温为30℃,黄檀素、黄檀素甲醚、4-甲氧基黄檀素检测波长分别为260、254、280 nm。结果黄檀素、黄檀素甲醚、4-甲氧基黄檀醌的平均回收率分别为98.24%、99.66%、99.06%,RSD值分别为1.57%、0.98%、1.29%。国内引种印度黄檀中黄檀素、黄檀素甲醚、4-甲氧基黄檀醌质量分数范围分别为0.756%~2.347%、0.059%~0.349%、0.047%~1.240%。结论本方法简便、准确,重复性、稳定性好,可用于印度黄檀中黄檀素、黄檀素甲醚和4-甲氧基黄檀醌的质量分数测定。Objective To establish an HPLC method for the quantitative determination of dalbergin,Omethyldalbergin and 4-methoxydalbergione in Dalbergia sissoo Roxb.Methods The separation was performed on Waters Symmetry C18column（ 250 mm× 4.6 mm,5 μm） eluted with a mobile phase of methanol-0.2%phosphoric acid solution in a gradient elution. The detection wavelength of dalbergin,O-methyldalbergin and4-methoxydalbergione were 260,254 and 280 nm,respectively.Results The average recoveries of dalbergin,Omethyldalbergin and 4-methoxydalbergione were 98.24%,99.66%,and 99.06%,with RSD of 1.57%,0.98%and 1.29%,respectively. The contents of dalbergin,O-methyldalbergin and 4-methoxydalbergione were in the range of 0.756%-2.347%,0.059%-0.349%,and 0.047%- 1.240% in the introduced Dalbergia sissoo Roxb.Conclusion The HPLC method is accurate and reproducible for the quantitative determinations of dalbergin,O-methyldalbergin and 4-methoxydalbergione in Dalbergia sissoo Roxb.

关 键 词：印度黄檀 高效液相色谱法 黄檀素 黄檀素甲醚 4-甲氧基黄檀醌 

分 类 号：R284.1[医药卫生—中药学]