UPLC/Q-TOF-MS技术快速鉴定瑶药别旁茶提取物的化学成分  

UPLC/Q-TOF-MS based rapid analysis and identification of chemical composition of Jasminum elongatum( Bergius) Willd.

作　　者：王凤云[1] 韩亮[1] 宋雨鸿[2] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]广州市第一人民医院中医科,广东广州510180

出　　处：《广东药学院学报》2016年第1期55-60,共6页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金资助项目(81503352)

摘　　要：目的采用UPLC/Q-TOF-MS技术快速分析和鉴定瑶药别旁茶的化学成分。方法采用AQUITY UPLC/Q-TOF micro联用仪,以0.1%(体积分数,下同)甲酸-乙腈为流动相梯度洗脱,ESI正负离子模式下采集数据,以Masslynx4.1软件分析数据,根据正、负离子质谱数据及元素组成分析,结合对照品和相关文献得出结果。结果共检测了29个色谱峰,鉴定了其中的26个化合物。结论 UPLC/Q-TOF-MS技术分析可获得别旁茶中各类化学成分丰富的结构信息,为这些化学成分的鉴定提供了一种快速有效的方法,为阐明别旁茶的药效物质基础提供了有力依据。Objective To make a qualitative analysis on chemical components in Jasminum elongatum（ Bergius） Willd. by UPLC/Q-TOF-MS. Methods AQUITY UPLC/Q-TOF micro system was employed with gradient elution of 0.1% formic acid-methyl cyanides as the mobile phase under the ESI positive and negative modes. Data was analyzed by masslynx 4. 1 software. Results 29 peaks were profiled and 26 compounds of J. elongatum were identified by UPLC-MS. Conclusion It provided an accurate and fast method to identify the main components from J. elongatum,as well as powerful support for clarifying the element composition.

关 键 词：别旁茶 UPLC/Q-TOF-MS 化学成分 

分 类 号：R29[医药卫生—民族医学] O657.63[医药卫生—中医学]