香鳞毛蕨有效部位对红色毛癣菌体外抗真菌的作用  

Antifungal effect of the active fraction of Dryopteris fragrans on Trichophyton rubrum in vitro

作　　者：黄奕曦 沈志滨[1] 江涛[2] 陈艳芬[1] 刘家媛 张莉莉[1] 唐春萍[1] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]广东药学院实验动物中心,广东广州510006

出　　处：《广东药学院学报》2016年第1期78-82,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：科技部"十二五"重大新药创制项目(2011ZX09102-007-03);广东省科技厅科技计划项目(2010B030700044;2011A030100013);广州市科技局科技计划项目(12A062131631);2015年广东省公益研究与能力建设专项资金项目(2015A030302088)

摘　　要：目的采用试管稀释法和CLSI M38-A2微量液基稀释法研究香鳞毛蕨有效部位（简称DF）对红色毛癣菌的体外抗真菌作用,并比较两种方法的一致性。方法利用两种方法测定DF对红色毛癣菌标准株（6株）和临床分离菌株（7株）的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。结果试管稀释法测定DF对13株红色毛癣菌的MIC范围为生药0.469~15.0 mg/m L;MFC范围为生药0.938~15.0 mg/m L。其中DF对标准菌株MIC范围为生药0.469~7.5 mg/m L,几何均值为2.652 mg/m L,MFC范围为生药0.938~15.0 mg/m L,几何均值为3.750 mg/m L。微量稀释法测定DF对红色毛癣菌标准菌株的MIC范围为生药0.312 5~5.0 mg/m L,几何均值为1.25 mg/m L;其MFC范围为生药1.25~20.0 mg/m L,几何均值为5.0 mg/m L。结论香鳞毛蕨有效部位对红色毛癣菌有抑菌作用和杀菌作用,两种方法的测定结果有较好的一致性。Objective To study the antifungal effect of the active fraction of Dryopteris fragrans（ L.） Schott（ DF） on Trichophyton rubrum by the test tube dilution and CLSI M38-A2 broth microdilution methods,and compare their consistency. Methods Two methods were used to detect the minimum inhibitory concentration（ MIC） and minimum fungicide concentration（ MFC） against Trichophyton rubrum＇s 6 standard strains and7 clinical isolates. Results The test tube dilution method determined the fungicidal activity of DF against Trichophyton rubrum,with the MIC values ranging from 0.469 to 15.0 mg / m L（ for the crude herb） and the MFC values ranging from 0.938 to 15.0 mg / m L. Among the Trichophyton rubrum＇s standard strains,the MIC values ranged from 0.469 to 7.5 mg / m L（ for the crude herb） and the geometric mean（ GM） value of MIC was 2.652 mg / m L,while the MFC values ranged from 0.938 to 15.0 mg / m L and the GM value of MFC was3. 750 mg / m L. The broth microdilution method also showed the fungicidal activity of DF against Trichophyton rubrum＇s standard strains. The MIC values ranged from 0.312 5 to 5.0 mg / m L（ for the crude herb） and the GM value of MIC was 1.25 mg / m L,while the MFC values ranged from 1.25 to 20.0 mg / m L and the GM value of MFC was 5. 0 mg / m L. Conclusion The active fraction of Dryopteris fragrans exerts antifungal effect against Trichophyton rubrum. Two detection methods show a proper consistency.

关 键 词：香鳞毛蕨 红色毛癣菌 试管稀释法 微量稀释法 最低抑菌浓度 最低杀菌浓度 

分 类 号：R285[医药卫生—中药学]