侧脑室微量注射GnIH对OEP大鼠血浆GnRH、LH水平的影响  

EFFECTS OF MICROINJECTION OF GNIH INTO LATERAL VENTRICLE ON GNRH AND LH LEVEL IN PLASMA OF OEP RATS

作　　者：苏玮[1] 魏萌[1] 冯超[1] 杨正江[1] 陈心悦[1] 乔跃兵[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《承德医学院学报》2016年第2期95-97,共3页Journal of Chengde Medical University

基　　金：河北省自然科学基金项目(H2013406115);河北省卫生厅医学科学研究项目(20130012);河北省人口计生委科学研究项目(2012-A22);国家卫生计生委计划生育与优生重点实验室开放课题(20150002)

摘　　要：目的:观察侧脑室注射促性腺激素抑制激素(Gn IH)对OEP大鼠血浆促性腺激素释放激素(Gn RH)和黄体生成素(LH)水平的影响。方法:健康成年雌性SD大鼠40只,均建立卵巢摘除兼予雌激素注射(OEP)模型。随机分为Gn IH实验组和生理盐水对照组,每组20只,大鼠分别给予侧脑室注射Gn IH(5μl)和同等剂量生理盐水。采用ELISA法检测注射后1h、2h、4h、6h(n=5)大鼠血浆Gn RH和LH的水平。结果:与对照组相比,注射Gn IH1h、6h Gn RH、LH水平无明显变化(P>0.05);注射Gn IH 2h、4h Gn RH、LH水平明显降低(P<0.05)。结论:侧脑室注射Gn IH可影响OEP大鼠血浆Gn RH和LH的水平,且影响作用与时间有关。Objective： To observe the effects of microinjection of gonadotropin-inhibitory hormone （GnIH） into lateral ventricle on gonadolrophin releasing hormone（GnRH） and luteinizing hormone （LH） level in plasma of OEP rats. Methods： 40 healthy adult female SD mrs were established OEP model and then randomly divided into normal saline control group and GnlH group. ELISA was used to detect the plasma GnRH and LH level 1h, 2h, 4h, 6h （each time point 5 rats） after respectively injected normal saline and GnIH （5μl） into the rats＇ lateral ventricle. Results： Compared with rats in control group, there was no significant changes in GnRH and LH level of rats in GnIH group lh and 6h after injection （P〉0.05）. The GnRH and LH level of rats in GnIH group 2h and 4h after injection were obviously lower than that of rats in control group （P〈0.05）. Conclusions： Microinjection of GnlH into lateral ventricle can affect the plasma GnRH and LH level of OEP rats; Moreover, these effects are related to time.

关 键 词：促性腺激素抑制激素(GnIH) 促性腺激素释放激素(GnRH) 黄体生成素(LH) 卵巢摘除兼予雌激素(OEP)大鼠 

分 类 号：R338.2[医药卫生—人体生理学]