束悗通经推拿疗法对糖尿病周围神经病变大鼠坐骨神经功能与SOD表达的影响研究  

Effect of the Traditional Tuina Therapy of Shumantongjing on Diabetic Peripheral Neuropathy in Rats Model of Sciatic Nerve of SNVC and SOD Expression

作　　者：陈林青[1] 刘明军[1] 卓越[1] 陈绍涛[1] 于明超[1] 仲丛文 李波[1] 王蕾[1] 张欣[1] 

机构地区：[1]长春中医药大学针灸推拿学院,长春130117

出　　处：《中国中医药现代远程教育》2016年第5期135-137,共3页Chinese Medicine Modern Distance Education of China

基　　金：国家中医药管理局中医学术流派传承工作室建设项目(No:20130101137JC);吉林省中医药管理局科学技术基金项目(No:2014-Q31);吉林省教育厅高校科学技术研究规划项目(No:2015-348)

摘　　要：目的观察"束悗通经疗法"对糖尿病大鼠坐骨神经功能和SOD表达的影响。方法将80只大鼠随机平均分为对照组、模型组、推拿组和药物组,采用腹腔注射链脉佐菌素的方法造模,观察各组动物坐骨神经传导速度(SNVC)和坐骨神经中超氧化物歧化酶(SOD)表达的变化情况。结果对各组动物坐骨神经SNVC检测发现,模型组与对照组比较,SNVC呈现显著性差异(P<0.01),推拿组与模型组比较,SNVC呈现显著性差异(P<0.01),药物组与模型组比较,SNVC呈现显著性差异(P<0.01),推拿组与药物组比较,SNVC无明显差异(P>0.05);对各组动物坐骨神经SOD检测发现,模型组与对照组比较,坐骨神经中SOD表达呈现显著性差异(P<0.01),推拿组与模型组比较,SOD表达呈现显著性差异(P<0.01),药物组与模型组比较,SOD表达无明显差异(P>0.05)。结论 "束悗通经推拿疗法"能够有效改善DPN大鼠模型下肢坐骨神经SNVC,表明对周围神经功能恢复具有积极的促进作用;同时"束悗疗法"能够提高坐骨神经中SOD的表达,表明具有抗氧化的作用从而发挥保护坐骨神经形态功能的疗效。本研究将为糖尿病周围神经病变提供新的辅助治疗手段,也为"束悗通经推拿疗法"的推广普及奠定实验室基础。Objective To observe the effect of the traditional Tuina therapy of Shuman Tongjing on diabetic peripheral neuropathy in rats model of sciatic nerve of SNVC and SOD expression. Methods 80 rats were randomly divided into control group, model group,Tuina group and drug group. The peritoneal injection of STC was used to prepare the rats of diabetic peripheral neuropathy. The SNCV of the sciatic nerve and the SOD in the sciatic nerve were observed. Results In the SNVC detection, SNVC showed a significant difference between the control group and the model group（ P0.01）, SNVC showed a significant difference between the tuina group and the model group（ P 0.01）, SNVC showed a significant difference between the drug group and the model group（ P 0.01）, there was no significant difference in SNVC between the Tuina group and the drug group（ P 0.05）. In the SOD expression detection, SOD expression showed a significant difference between the control group and the model group（ P 0.01）,SOD expression showed a significant difference between the Tuina group and the model group（ P0.01）, SOD expression showed no significant difference between the drug group and the model group（ P0.05）. Conclusion The traditional Tuina therapy of Shuman Tongjing can effectively improve the SNVC of sciatic nerve, and it showed that Tuina has positive effects on peripheral nerve functional recovery. Tuina therapy of Shuman Tongjing can improve the expression of SOD in sciatic nerve, and it showed that Tuina has antioxidant effect so as to play a protective efficacy of morphology and function of sciatic nerve. This study will provide a new means for the treatment of diabetic peripheral neuropathy, and will lay the foundation for the promotion of the traditional Tuina therapy of Shuman Tongjing.

关 键 词：推拿 束悗 糖尿病 周围神经病变 SOD表达 消渴 动物实验 

分 类 号：R244.1[医药卫生—针灸推拿学]