迷迭香酸对CHO细胞DNA损伤的防护作用  

The Protective Effect of Rosemary Acid on DNA Damage of CHO Cells

作　　者：夭建华[1,2] 高茜[2] 李雪梅[2] 黄海涛[2] 管莹[2] 米其利[2] 罗瑛[1] 

机构地区：[1]昆明理工大学,云南昆明650504 [2]云南烟草科学研究院,云南昆明650106

出　　处：《香料香精化妆品》2016年第1期33-36,共4页Flavour Fragrance Cosmetics

基　　金：云南中烟工业有限责任公司资助项目(编号2015JC02)

摘　　要：采用高内涵分析技术测定DNA损伤/双键断裂的标志物γH2AX,来研究迷迭香酸对细胞DNA损伤的防护作用。即通过在体外对CHO细胞施予迷迭香酸,再诱导DNA损伤,对致损后的细胞进行γH2AX及Hoechst双荧光染色标记,最后通过分析γH2AX的荧光强度从而对细胞DNA损伤程度进行半定量检测。结果发现,施予迷迭香酸的细胞与对照相比较,DNA损伤程度降低,表明迷迭香酸对CHO细胞的DNA损伤具有防护作用。To investigate the protective effect of rosemary acid on DNA damage,the DNA damage/double strand breaks biomarker γH2AX was detected by using high content analysis technique. CHO cells were treated with rosemary acid, and then DNA damage was induced. Cells were double staining with γH2AX and Hoechst, and finally the obtained fluorescence intensity of γH2AX was used to semi- quantitatively characterize the degree of DNA damage. The results showed that comparing with the control, CHO cells treated with rosemary acid had a lower degree of DNA damage, which indicated that the rosemary acid had protective effect on the DNA damage of CHO cells.

关 键 词：CHO细胞 迷迭香酸 高内涵分析技术 γH2AX 

分 类 号：R285[医药卫生—中药学]