衰老对AhR及其血管生成相关因子表达的影响  

Effects of Aging on the Expression of Ah R and Angiogenesis Related Factors

作　　者：荣棣君[1] 黄婧娟[1] 李瑞琳[1] 方根强[1] 段俊丽[1] 

机构地区：[1]上海交通大学医学院附属新华医院老年医学科,上海200092

出　　处：《现代生物医学进展》2016年第5期806-810,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(11374213);中国科学院红外物理国家重点实验室开放项目(200901)

摘　　要：目的:探讨芳香烃受体(aryl hydrocarbon receptor,Ah R)在过氧化氢(H_2O_2)诱导的人脐静脉内皮细胞(HUVECs)衰老模型中表达的变化情况及其对血管生成相关因子表达的影响。方法:采用不同浓度的H_2O_2(100μM,200μM,300μM)处理HUVECs诱导内皮细胞衰老,通过β-半乳糖苷酶染色观察细胞衰老情况,Western blot检测HUVECs中Ah R蛋白以及血管生成相关因子低氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)蛋白表达,ELISA检测细胞培养上清液中血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的变化。结果:与对照组相比,经过不同浓度H_2O_2处理后,HUVECs均出现细胞衰老表现,Ah R蛋白表达显著增加,HIF-1α以及VEGF蛋白表达明显减少,且均呈现出剂量依赖性,以300μM H_2O_2处理组最为显著。结论:在过氧化氢诱导衰老的人脐静脉内皮细胞中Ah R蛋白表达明显增加,HIF-1α和VEGF等促血管生成因子明显减少。Objective： To investigate the expression change of aryl hydrocarbon receptor（aryl hydrocarbon receptor, AhR） in hydrogen peroxide（H2O2） induced senescence and AhR effects on angiogenesis related factor in senescent human umbilical vein endothelial cells（HUVECs）. Methods： Senescence induced by different concentrations of H2O2（100 μM, 200 μM, 300 μM） in HUVECs was examined by β-galactosidase staining. Protein expression of AhR and hypoxia-inducible factor-1α（HIF-1α） were analysed by Western blot.The concentrations of vascular endothelial growth factor（VEGF） in culture medium were tested by ELISA. Results： Compared with the control group, all of the H2O2 groups showed signs of senescence. Meanwhile, H2O2 significantly increased the AhR protein expression and decreased the protein expression of HIF-1α and VEGF in a dose-dependent manner in HUVECs. Those changes were most obviously in 300μM treatment group. Conclusion： Protein expression of AhR up regulated in H2O2-induced senescent HUVECs, as well as angiogenesis related factors（HIF-1α, VEGF） decreased significantly.

关 键 词：芳香烃受体 人脐静脉内皮细胞 衰老 低氧诱导因子-1Α 血管内皮生长因子 

分 类 号：Q419[生物学—生理学] R33[医药卫生—人体生理学]