H_2S对心跳骤停复苏大鼠脑血流动力学及神经功能的影响  

Effects of H_2S on Cerebral Hemodynamic Parameters and Neurological Function in Rats after Cardiac Arrest and Resuscitation

作　　者：陈炬辉[1] 程龙[1] 李航兵[1] 孙永鑫[1] 张兵[1] 

机构地区：[1]哈尔滨医科大学附属第二医院麻醉科,黑龙江哈尔滨150001

出　　处：《现代生物医学进展》2016年第6期1012-1017,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金面上项目(81372026)

摘　　要：目的:探讨吸入外源性H_2S对心跳骤停复苏大鼠脑血流动力学及神经功能的影响。方法:选择成年雄性SD大鼠18只,随机分为假手术组(sham,n=6)、窒息导致心跳骤停复苏组(CA,n=6)、心跳骤停复苏+H_2S组(H_2S,n=6),观察H_2S对心跳骤停复苏大鼠脑血流的影响。选择成年雄性SD大鼠45只,随机分为sham组(n=5)、CA(n=20)和H_2S组(n=20),观察H_2S对心跳骤停复苏大鼠神经功能和存活率的影响。结果:CA组与H_2S组在ROSC后MAP和CPP均快速上升,远高于基础值(P<0.05);而后两组MAP和CPP均缓慢下降,实验结束时,明显低于基础值(P<0.05)。两组间比较差异无统计学意义(P>0.05)。CA组与H_2S组CBF曲线在ROSC后上升至峰值,随后逐渐下降,两组在峰值无明显差异,但H_2S组CBF减少量显著少于CA组(P<0.05)。而在CVR曲线,在实验最后,CA组CVR值仍明显高于基础值(P<0.05),而H_2S组基本降至基础值,明显低于CA组(P<0.05)。H_2S组的14天存活率(80%)明显高于CA组(50%,P<0.05)。在胶带移除实验(tape removal test,TRT)所需时间上,CA组与H_2S组在第1天、第3天及第14天都存在显著差异(P<0.05)。H_2S组第14天海马CA1区神经元存活数(33±8)明显多于CA组(20±6,P<0.05),但仍较假手术组少(53±10,P<0.05)。结论:外源性H_2S吸入对大鼠心跳骤停复苏后MAP及CPP无明显影响。外源性H_2S通过降低CVR,改善CBF,从而改善大鼠心跳骤停复苏后的脑循环。外源性H_2S能明显提高心跳骤停复苏后大鼠的存活率,降低TRT所需时间,同时显著增加海马CA1区神经元的存活,改善大鼠心跳骤停复苏后神经功能转归。Objective：To investigate the effects of exogenous hydrogen sulfide（H_2S） on cerebral hemodynamic parameters and neurological function in rats after cardiac arrest（CA） and resuscitation.Methods：18 adult male SD rats were randomly divided into three groups：sham group（sham,n = 6）,cardiac arrest group（CA,n = 6）,cardiac arrest ＋ H_2S group（H_2S,n = 6）.To observe the effects of H_2S on cerebral hemodynamic in rats after cardiac arrest and resuscitation.45 adult male SD rats were randomly divided into three groups：sham group（n=5）,CA group（n = 20）,H_2S group（n = 20）.To observe the effects of H_2S on survival rate and neurological function in rats after CPR.Results：There was an abrupt and transient increase in MAP and CPP after ROSC in both CA group and H_2S group,which was significantly greater than the baseline value（P 0.05）;After that,MAP and CPP decreased gradually,at the end of the experiment both of which were significantly lower than baseline values（both P 0.05）.There were no significant differences in MAP and CPP between the CA group and the H_2S group at any point during the experiment.After ROSC,the CBF increased significantly then decreased gradually in both CA group and H_2S group,but the decrease in CBF in H_2S group was significantly less than that of the CA group（P 0.05）.The CVR decreased slowly in the CA group in the end,which was still significantly higher than the baseline value（P 0.05）.Finally the CVR in H_2S group almost decreased to baseline value,which significantly lower than that of the CA group（P 0.05）.There was a significant difference in the 14-d survival rate between the CA group（50%）and H_2S（80%）group（P 0.05）.Compared with the CA group,the TRT were significantly decreased in H_2S group at day 1,day 3 and day 14 after ROSC（P 0.05）.For comparison,the number of viable neurons in the H_2S group（33 ± 8） was significantly more than in the CA group（20 ± 6,P 0.05）,but still less than that in the sham group（P 0.

关 键 词：H2S 脑血流 神经功能 心跳骤停 

分 类 号：R541.78[医药卫生—心血管疾病] R-33[医药卫生—内科学]