SPA患者循环EPCs及SDF-1α与冠状动脉侧支循环的相关性分析  

Correlation Analysis of EPCs,SDF-1α and Collateral Circulation in SAP Patients With Serious Coronary Artery Stenosis

作　　者：朱凯[1] 缪绯[1] 游伟[1] 张杰波 刘映峰[1] 

机构地区：[1]南方医科大学珠江医院心血管内科,广东广州510280

出　　处：《现代生物医学进展》2016年第6期1051-1053,1060,共4页Progress in Modern Biomedicine

摘　　要：目的:研究冠状动脉严重狭窄稳定型心绞痛(Stable angina pectoris,SPA)患者循环内皮祖细胞(endothelial progenitor cells,EPCs)及基质细胞衍生因子(SDF)-1α与冠状动脉侧支循环(CCC)形成的相关性,以期为治疗冠心病提供新的思路。方法:选择2012年8月到2014年12月在我院就诊的88例冠状动脉严重狭窄的稳定型心绞痛患者(CCC良好40例、不良48例),均采集外周血测定EPC数量、体外生成血管能力,并用ELISA法检测其血浆SDF-1α水平,采用直线相关和Pearson检验分析CCC良好与不良者各指标间及与CCC分级的相关性;将所有入选病例随机分为6组,并分离外周血单个核细胞并分别加入不同的培养液,培养7天后体外测定EPCs数量以及生成血管的能力,并通过ELISA法检测培养液上清中VEGF的蛋白水平。结果:CCC不良组EPCs数量、体外生成血管能力及SDF-1α水平均明显低于CCC良好组(P<0.05)。体外生成血管能力、循环EPCs数量以及SDF-1α水平均与CCC分级呈现显著的正相关性(r=0.72、0.67、0.79,均P<0.05);循环EPCs数量、SDF-1α水平以及体外生成血管能力亦均呈现显著正相关性(r=0.78、0.62,均P<0.05)。与PBS、SDF-1α+AMD3100及SDF-1α+KI8751干预物质比较,SDF-1α能够呈剂量依赖性的明显提高EPCs数量、增强其体外生成血管的能力及VEGF水平(P<0.05)。结论:冠状动脉严重狭窄稳定型心绞痛患者循环EPCs及SDF-1α与CCC形成有关,VEGF可能参与该过程。Objective：To study the correlation between coronary collateral circulation（CCC） and endothelial progenitor cells（EPCs）,stromal cell derived factor-1α（SDF-1α） in stable angina pectoris（SAP） patients with serious coronary stenosis,and to provide new thread to coronary artery disease.Methods：88 SAP patients with serious coronary artery stenosis of August 2012 to December 2014 in our hospital were selected,in which 40 patients with well CCC and 48 patients with poor CCC.EPCs were isolated from peripheral blood samples,then its colonies were counted and vascular genesis capacity in vitro was assayed respectively.The plasma level of SDF-1α was determined by ELISA.Rectilinear correlation and Pearson analysis were adopted to analyze the correlation of above indexes and CCC classification.MNC were isolated from peripheral blood samples of 88 patients.All selected cases randomly divided into 6groups and then isolated peripheral blood mononuclear cells with addition of different culture solution.EPC colonies were counted and vascular genesis capacity were assayed respectively,and vascular endothelial growth factors from the culture medium were also measured In vitro ELISA after 7 days of cultivation.Results：CCC well group exhibited an increased number of colony-forming units（CFUs）,vascular genesis in vitro and level of SDF-1α,compared with CCC poor group.There was Significant positive correlation between the 3 indexes and the classification of CCC（P0.05）.Compared with groups of PBS,SDF-1α＋AMD3100,SDF-1α＋KI8751,the number of CFUs and vascular genesis capacity in vitro of cultured EPCs,concentration of VEGF in the culture medium were increased with a dose-dependent manner by SDF-1α（P0.05）.Conclusion：The development of CCC in SAP patients with serious coronary artery stenosis is correlated with EPCs and the level of SDF-1α,and this procedure maybe regulated by VEGF.

关 键 词：冠状动脉严重狭窄 稳定型心绞痛 内皮祖细胞 基质细胞衍生因子-1Α 冠状动脉侧支循环 

分 类 号：R541.4[医药卫生—心血管疾病]