新疆维、汉妇女宫颈脱落细胞中hTERC基因表达  

Expression of hTERC gene in Cervical Cell between Uygur and Han Women in Xinjiang

作　　者：马玉兰[1,2] 玛依努尔.尼牙孜 朱开春[2] 赵丽霞[2] 开丽比努尔.依马木 

机构地区：[1]新疆医科大学,新疆乌鲁木齐830054 [2]新疆维吾尔自治区人民医院,新疆乌鲁木齐830054

出　　处：《现代生物医学进展》2016年第6期1139-1143,共5页Progress in Modern Biomedicine

基　　金：新疆维吾尔自治区人民医院院内基金项目(20100107);国家自然科学基金项目(81360317)

摘　　要：目的:研究hTERC基因在新疆维吾尔族和汉族妇女宫颈脱落细胞中的表达情况及差异性。方法:收集2012年9月至2014年3月在新疆维吾尔自治区人民医院妇科门诊就诊或行宫颈癌机会性筛查的新疆维吾尔族和汉族妇女病例1160例,选择其中HPV感染阳性或TCT阳性或两项同时阳性的465例纳入研究队列,通过F1SH技术检测宫颈脱落细胞中hTERC基因的表达情况。结果:新疆维吾尔族与汉族妇女hTERC基因扩增的总阳性率无显著差异(P=0.620);维吾尔族与汉族妇女正常或慢性炎症组、CIN1组、CIN2组、CIN3组和SCC组hTERC基因扩增的阳性率亦均无显著差异(P=0.918,0.912,0.821,0.922,1.000),hTERC基因的表达率不存在民族差异;维吾尔族与汉族妇女正常或慢性炎症组/CIN1组和CIN2+组基因拷贝数的分布均无显著差异(P=0.925,0.862),且其正常或慢性炎症组/CIN1组和CIN2+组TERC:CEP比例分布亦均无显著差异(P=0.986,P=0.979),hTERC基因的扩增类型不存在民族差异;维吾尔族和汉族妇女hTERC基因扩增阳性率的大小顺序均为:SCC组≈CIN 3组>CIN 2组>CIN 1组≈正常或慢性炎症组,随着宫颈病变恶性程度的增加,各组中hTERC基因的扩增阳性率有增加的趋势,hTERC基因的表达与宫颈病变的恶性程度呈正相关(相关系数=0.648和0.712,P<0.001)。结论:新疆维吾尔族与汉族妇女hTERC基因的表达率不存在民族差异;维吾尔族与汉族妇女hTERC基因的扩增类型不存在民族差异;随着宫颈病变恶性程度的增加,维吾尔族和汉族妇女hTERC基因的扩增阳性率有增加的趋势,hTERC基因的表达与宫颈病变的恶性程度呈正相关。Objective：To study the expression and differentiation of hTERC gene in cervical cell between Uygur and Han women in Xinjiang.Methods：From September 2012 to March 2014,1160 cases that consulted a doctor or had an opportunistic screening of cervical cancer were collected in the department of Xinjiang Uygur Autonomous Region People＇s Hospital.All the cases were examined with Thin Prep Cytology Test（TCT） and HPV genotyping,and 465 cases with HPV infection positive or TCT positive or the both positive were included in the study cohort.All the cases included in the study cohort underwent colposcopy and biopsy,and the expression of hTERC gene was detected with fluorescence chromosomal in situ hybridization（FISH）.Results：The total positive rate of hTERC gene amplification had no significant difference between Uygur and Han women（P=0.620）,and the positive rate of hTERC gene amplification in normal or chronic inflammation group,CIN1 group,CIN2 group,CIN3 group and SCC group had no significant difference between Uygur and Han women（P=0.918,0.912,0.821,0.922,1.000）,and the gene copy number（P=0.925,0.862）and distribution ratio（TERC：CEP）（P=0.986,P=0.979）of normal or chronic inflammation group /CIN1 group and CIN2＋group had no significant difference between Uygur and Han women.The order of the positive rate of hTERC gene amplification was SCC group ≈CIN3 group CIN2 group CIN1 group ≈ normal or chronic inflammation group,and hTERC gene expression was positively correlated with the malignancy of cervical lesions（rs=0.648 and 0.712,P0.001）.Conclusions：The expression of hTERC gene had no significant difference between Uygur and Han women in Xinjiang,and hTERC gene expression was positively correlated with the malignancy of cervical lesions.

关 键 词：宫颈病变 HTERC基因 维汉妇女 

分 类 号：R711.74[医药卫生—妇产科学]