荔枝果皮对不同致病力炭疽菌侵染的结构变化差异  

Difference of structural change of litchi pericarp infected by different pathogenicity strains of Colletotrichum

作　　者：张新春[1] 彭元科 高兆银[1] 王家保[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《广东农业科学》2015年第24期104-106,I0001,I0002,共5页Guangdong Agricultural Sciences

基　　金：国家现代农业产业技术体系专项:国家荔枝龙眼产业技术体系(CARS-33-02);海南省科技厅重点实验室和工程技术研究中心平台建设项目(ZDZX2013023-1)

摘　　要：用两个强弱不同致病力炭疽菌株0977-19-2和09619-1-1接种无核荔枝果皮,以探明荔枝果实接种炭疽菌后的结构变化差异,明晰不同致病力炭疽菌的致病机制。结果显示,接种强致病力菌株0977-19-2后,果皮结构变化迅速,侵染1 d后菌丝即可穿过表皮细胞角质层,5 d后表皮组织内产生大量分生孢子盘和分生孢子,果皮表面凹陷处细胞消融,几无正常细胞;接种弱致病力菌株09619-1-1后的前2 d,外果皮和栅状组织几乎没有受到损害,5 d后凹陷处细胞大量消融,部分海绵组织细胞消融,果皮表面产生较多的菌丝体和少量分生孢子。表明荔枝果皮接种不同致病力胶孢炭疽菌后,果皮结构对强致病力菌株的反应迅速,寄主细胞消解、病原菌侵入、定殖和扩展的速度都明显较快。In order to study the structural change of litchi pericarp infected by Colletotrichum,we used two different pathogenicity of C. gloeosporioides strains,0977-19-2 with strong pathogenicity and 09619-1-1 with weaker pathogenicity,to infect ‘wuhe＇ fruits. The results showed that the structure of pericarp infected by 0977-19-2 changed rapidly, the hypha penetrated the epidermal cells after 1 day,lots of acervulus and conidiums were produced among epidermal cells after 5 days,and acervulus spreaded gradually. The depressed cell of pericarp were dissolved,and none normal cell was existed. The epicarp and palisade tissue treated by 09619-1-1,did not been damaged in 2 days. In the last day,most of cells in sunked pericarp disappeared,and part of spongy tissue cells dissolved. Mycelia and conidiospores were on pericarp. There were significant different responses in pericarp after the infection of different pathogenicity of Colletotrichum. The response on strong pathogenicity was quick,the cells melted rapidly,the speed of invading,colonizing and spreading of strong pathogenicity of Colletotrichum were faster.

关 键 词：荔枝 炭疽菌 侵染 结构 

分 类 号：S436.671[农业科学—农业昆虫与害虫防治]