回回甘松饮含药血清对高糖诱导的肾小球系膜细胞外基质堆积的影响  

Effects of Hui-hui Gan-song Yin on accumulation of extracellular matrix of glomerular mesangial cells induced by high glucose

作　　者：南一[1,2] 马丽杰[1] 袁玲[2,3,4,5] 

机构地区：[1]宁夏医科大学中医学院,宁夏银川750004 [2]宁夏医科大学回医药现代化省部共建教育部重点实验室,宁夏银川750004 [3]宁夏医科大学药学院,宁夏银川750004 [4]宁夏回药现代化工程技术研究中心,宁夏银川750004 [5]宁夏回医药协同创新中心,宁夏银川750004

出　　处：《中国生化药物杂志》2015年第11期15-18,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：2012国家自然科学基金(No.81202774)

摘　　要：目的探讨回回甘松饮(Hui-hui Gan-song Yin,HGY)含药血清对高糖诱导的大鼠肾小球系膜细胞(mesangial cells,MCs)外基质堆积的影响。方法健康雄性SD大鼠40只随机分为正常对照组(蒸馏水)、格列喹酮组(10 mg/kg)、HGY(5、10 g/kg)组,每组各10只,每天给药2次,连续3 d后制备含药血清用于体外培养大鼠MCs,分为正常对照组(10%正常对照组大鼠血清)、高糖组(30 mmol/L葡萄糖+10%正常大鼠血清)、格列喹酮组(30 mmol/L葡萄糖+10%格列喹酮组大鼠血清)、HGY高剂量组(30 mmol/L葡萄糖+10%HCY高剂量组大鼠血清)、HGY低剂量组(30 mmol/L葡萄糖+10%HCY低剂量组大鼠血清)。细胞培养结束后,采用Western blot方法检测纤维连接蛋白(fibronectin,FN)、Ⅰ型胶原蛋白(ColⅠ)及Ⅳ型胶原蛋白(ColⅣ)含量。结果与空白对照组比较,高糖组FN、ColⅠ及ColⅣ表达量上调(P<0.01);HGY含药血清可降低在高糖诱导的MCs中FN、ColⅠ及ColⅣ表达量(P<0.05)。结论 HGY含药血清可降低MCs在高糖环境下的FN、ColⅠ及ColⅣ蛋白表达水平,从而减少了ECM堆积,保护肾小球,延缓糖尿病肾病进程。Objective To investigate the effects of Hui-hui Gan-song Yin（ HGY） on the accumulation of extracellular matrix of rat glomerular mesangial cells（ MCs） induced by high glucose. Methods The 40 SD rats were randomly divided into normal control group（ distilled water）,glurenorm group（ 10 mg / kg）,HGY high-dose group（ 10 g / kg） and HGY low-dose group（ 5 g / kg）,10 rats in each group. The rats in each group were treated with corresponding drugs,twice a day. After 3 days,the serum containing each drug were prepared to culture rat MCs in vitro. The MCs were divided into the normal control group（ 10% serum of rats in normal control group）,high glucose group（ 30 mmol / L glucose ＋ 10% serum of rats in normal control group）,glurenorm group（ 30 mmol / L glucose ＋ 10% serum of rats in glurenorm group）,HGY high-dose group（ 30 mmol / L glucose ＋ 10% serum of rats in HGY high-dose group） and HGY low-dose group（ 30 mmol / L glucose ＋ 10% serum of rats in HGY low-dose group）. The fibronectin（ FN）,ColⅠand ColⅣ levels were detected by Western blot. Results Compared with normal control group,the expression of FN,ColⅠand ColⅣ in high glucose group increased（ P 0. 01）. The HCY suppressed the protein expression of FN,Col Ⅰ and Col Ⅳ significantly（ P 0. 05）. Conclusion The serum containing HGY could suppressed protein expression of FN,ColⅠand ColⅣ and inhibit the accumulation of extracellular matrix of MCs induced by high glucose,which could protect glomerulus and delay the development of diabetic nephropathy.

关 键 词：回回甘松饮 肾小球系膜细胞 细胞外基质堆积 纤维连接蛋白 ColⅠ ColⅣ 

分 类 号：R692.6[医药卫生—泌尿科学]