脐带间充质干细胞对博来霉素诱导实验性小鼠肺纤维化模型影响的研究  

Study on effect of UC-MSC in IPF experimental model induced by bleomycin in mice

作　　者：冯丽君[1] 张颖超[1] 

机构地区：[1]天津医科大学宝坻临床学院呼吸科,天津301800

出　　处：《中国生化药物杂志》2015年第11期19-21,25,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的探讨脐带间充质干细胞(umbilical cord-mesenchymal stem cells,UC-MSC)对博来霉素诱导的实验性小鼠肺纤维化中羟基脯氨酸(hydroxyprine,HYP)、活性氧(superoxyde dismutase,SOD)及丙二醛(malonaldehyde,MDA)的含量的影响。方法采用小鼠气管内注人博来霉素诱导实验性小鼠肺纤维化,取第四代UC-MSC细胞通过尾静脉注射干预,给药21 d后,处死小鼠获取肺组织,对其进行病理学切片观察以及检测组织中HYP、SOD及MDA的含量来评价UC-MSC对小鼠肺纤维化的影响。结果病理学染色结果表明,治疗组(给予博来霉素+UC-MSC)较模型组(仅给予博来霉素)相比,治疗组不仅可降低炎症细胞的浸润以及胶原沉积等病理特征,还可降低由博来霉素诱导实验性小鼠肺纤维化模型中HYP(P<0.01)和MDA(P<0.05)的含量以及增高SOD的含量(P<0.01)。结论 UC-MSC可对由博来霉素诱导的实验性小鼠肺纤维化具有一定的保护作用。Objective To investigate protective of umbilical cord-mesenchymal stem cells（ UC-MSC） on idiopathic pulmonary fibrosis（ IPF）experimental mode was induced by bleomycin in mice. Methods IPF experimental mode induced by bleomycin with intratracheal injection,and then the fourth generation UC-MSC was injected in caudal vein. The lung tissues were obtained to evaluate the influence of UC-MSC on IPF by the observation of histological evaluation（ HE） and Masson staining,the detection content of hydroxyprine（ HYP）,superoxyde dismutase（ SOD） and malonaldehyde（ MDA）. Results The HE and Masson staining showed that bleomycin ＋ UC-MSC group could alleviate inflammatory cell infiltration and pulmonary interstitial collagen deposition. bleomycin ＋ UC-MSC group could decline the content of HYP（ P〈0. 01）,MDA（ P〈0. 05） and improve the content of SOD（ P〈0. 01） compared with bleomycin group. Conclusion UC-MSC exhibits an protective effect on IPF experimental mode induced by bleomycin in mice.

关 键 词：脐带间充质干细胞 博来霉素 肺纤维化 

分 类 号：R285.5[医药卫生—中药学]