红杜仲的美白活性及其途径研究  

Study on the Whitening activity and pathway of red eucommia herbs

作　　者：林锦彬[1,2] 任桐[3] 连一江 李伟杰[1,2] 金鑫[1,2] 夏智波[1,2] 李文君[1,2] 

机构地区：[1]厦门大学医学院,福建厦门361102 [2]厦门市手性药物重点实验室,福建厦门361102 [3]华侨大学生物医学学院,福建泉州362021

出　　处：《中国生化药物杂志》2015年第12期21-23,28,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究红杜仲的不同乙醇浓度醇提物对黑色素瘤B16细胞系酪氨酸酶活性、黑素合成的影响,初步探讨红杜仲作为皮肤美白剂或色素沉积药物的可行性。方法以不同浓度乙醇进行回流分别获得30%、50%、70%、95%乙醇提取的得到4种红杜仲醇提物(PEE30、PEE50、PEE70、PEE95),选取对蘑菇酪氨酸酶抑制活性最强的提取物;再以B16造模,采用MTS法、Na OH裂解法、L-DOPA氧化法分别测定其对B16细胞的影响;Western blot检测PEE作用后Tyrosinase、TRP-1蛋白表达水平的变化。结果各红杜仲醇提物在体外均具有抑制蘑菇酪氨酸酶的作用,效果最强的为PEE70。在不影响B16细胞的浓度内,PEE70明显降低B16细胞酪氨酸酶活性和黑素的合成量(P<0.001)。Western blot结果表明,PEE70能够显著抑制黑素合成相关蛋白Tyrosinase、TRP-1的表达。结论 PEE能直接抑制B16细胞黑色素的合成,可用于美白产品或者色素沉积药物的开发,且上述变化通过抑制酪氨酸酶活性来完成。Objective To determine the impact of PEE with different ethanol concentration extract on the activity of tyrosinase in B16 melanoma and to explore the feasibility of PEE used as skin whitening agent make use of a model organism. Methods 30%,50%,70%,95%,different concentration of ethanol reflux got four kinds of PEE alcohol extract,respectively. And selected the most active inhibition to mushroom tyrosinase（ PEE30、PEE50、PEE70、PEE95）. The effect of extracts from the selected on B16 cell model was determined by MTS,Na OH splitting decomposition,LDOPA oxidation progress. The change in protein expression level after ethanol extract of PEE was determined by Western bolt. Results The capacity of inhibition to mushroom tyrosinase in each concentration of PEE extraction,but PEE70 had the most active inhibition. When challenged with B16 cell model,PEE70 showed the capacity of decrease in tyrosinase activities and melanin synthesis（ P〈0. 01） and had a does dependence; it also decreased Tyrosinase and TRP-1 expression compared with control B16 cells. Conclusion These data support the idea that PEE can restrain melanogenesis,through its inhibitory effect on the activity of tyrosinase.

关 键 词：红杜仲 B16细胞 黑素合成 抑制作用 

分 类 号：R285[医药卫生—中药学]