胸腺肽对哮喘大鼠的调节及机制的研究  

Research of thymosin on adjustment and mechanism in asthmatic mouse

作　　者：闫超[1] 许珂玉[1] 

机构地区：[1]辽宁医学院生物化学与分子生物学教研室,辽宁锦州121000

出　　处：《中国生化药物杂志》2015年第12期32-35,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：辽宁省教育厅基金资助项目(2009A469)

摘　　要：目的观察胸腺肽对哮喘大鼠Th1/Th2的调节作用,研究胸腺肽对T-bet和GATA-3表达的影响。方法将60只SPF级6~8周龄的雄性大鼠分为正常组、哮喘组、地塞米松组（0.5 mg/kg）、胸腺肽高剂量组（5 mg/kg）、胸腺肽中剂量组（2.5 mg/kg）、胸腺肽低剂量组（1.25 mg/kg）。用卵清蛋白致敏法建立大鼠哮喘模型。正常组及哮喘组腹腔注射生理盐水,其他组注射相应药物。用酶联免疫吸附试验（ELISA）法检测各组大鼠血清中IL-4、IFN-γ的含量。免疫组化法检测各组的肺组织中GATA-3和T-bet因子蛋白表达情况。结果哮喘组血清中IL-4含量比其他组浓度均高（P〈0.05）,哮喘组血清中IFN-γ含量比其他组浓度均低（P〈0.05）。哮喘组GATA-3蛋白的表达比其他组浓度均高（P〈0.05）。各组中T-bet的表达,哮喘组Fbet的表达比其他组浓度均低（P〈0.05）。结论胸腺肽通过上调T-bet和下调GATA-3实现上调IFN-γ和抑制IL-4的调节,对哮喘大鼠有治疗作用。Objective To explore the regulation role and expression level of thymosin for Th1 /Th2 and T-bet,GATA-3 in asthma model rats respectively. Methods 60 SPF grade male rats（ 6-8w） were randomly divided into six groups： control group,asthma model group,dexamethasone（ 0. 5mg / kg） group,thymosin high dose group（ 5 mg / kg）,thymosin middle dose group（ 2. 5 mg / kg）,thymosin low dose group（ 1. 25 mg / kg）. The asthma model was constructed with ovalbumin. The rat in the control group and asthma model group were injected with normal saline by intraperitoneal,while other rats were injected with corresponding drugs. Then the concentration of IL-4 and IFN-γ in the serum of rats were tested by the method of enzyme linked immunosorbent assay（ ELISA） and the protein expression level of GATA-3 and T-bet factor in the lung tissue were tested by immunohistochemistry in each group. Results The concentration of IL-4 in the serum was higher and IFN-γ was lower in the asthma model group than the other groups,while the protein expression level of GATA-3 was higher and T-bet was lower in the asthma model group than the other groups（ all P〈0. 05）.Conclusion Thymosin upregulation of IFN-γ and inhibition of IL-4 may be achieved by upregulating T-bet and downregulating GATA-3,and thymosin has therapeutic effect on asthma rats.

关 键 词：支气管哮喘 胸腺肽 GATA-3 T-BET 

分 类 号：R562.25[医药卫生—呼吸系统]