LC/MS/MS鉴定莲子心生物碱在Caco-2细胞中的代谢产物  

Metabolites Identification for Alkaloids from Nelumbinis Plumula in Caco-2 Cells by LC / MS / MS

作　　者：林敏婷[1] 张超[1] 唐思丽 赵中振[2] 陈虎彪[2] 张建业[1] 

机构地区：[1]广州医科大学药学院,广东广州510182 [2]香港浸会大学中医药学院

出　　处：《中药材》2015年第12期2531-2534,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(81473320);广东省科学技术厅-广东省中医药科学院联合科研专项(2012A032500021);广州市属高校科技计划项目(1201410039)

摘　　要：目的：应用LC/MS/MS鉴定去甲乌药碱、莲心碱、异莲心碱、荷花碱4种莲子心生物碱在Caco-2细胞中的代谢产物,推测其代谢途径。方法：100μmol/L生物碱作用Caco-2细胞3～12 h后收集并纯化样品,采用LC/MS/MS分析鉴定代谢产物。结果：去甲乌药碱的代谢产物有甲基化去甲乌药碱和去甲乌药碱葡萄糖醛酸苷;莲心碱和异莲心碱的代谢产物分别是去甲基莲心碱和去甲基异莲心碱;荷花碱的主要代谢物为莲心碱、异莲心碱和它们的进一步去甲基产物。结论：LC/MS/MS操作简便,可快速鉴定Caco-2细胞中的莲子心生物碱的代谢产物。莲子心生物碱主要代谢途径有甲基化、去甲基化和葡萄糖醛酸化。Objective： To identify the metabolites of norcoclaurine,liensinine,isoliensinine and neferine in Caco-2 cells by LC /MS / MS. Methods： After Caco-2 cells were treated with norcoclaurine,liensinine,isoliensinine or neferine for 3,6 and 12 h,samples were collected,purified and then analyzed by LC / MS / MS. The structures of the metabolites were elucidated by molecular masses,retention times,MS and MS / MS spectra comparing with those of the parent drug. Results： The procedure identified that the major metabolites of norcoclaurine were methylnorcoclaurine and norcoclaurine-glucuronide,the major metabolite of liensinine was demethyl-liensinine,the major metabolite of isoliensinine was demethyl-isoliensinine,the major metabolites of neferine were liensinine,isoliensinine and their further demethylation products. Conclusion： LC / MS / MS is simple,rapid and sensitive for the metabolites identification. Methylation,demethylation and glucuronidation are main metabolic pathways of alkaloids from Nelumbinis Plumula in Caco-2 cells.

关 键 词：LC/MS/MS 生物碱 莲子心 代谢产物 CACO-2细胞 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]