归芪聪志汤对VD大鼠模型脑组织热休克蛋白表达的影响  

Effect of Guiqicongzhi Decoction on Expression of HSP27 and HSP70 in Brain Tissue of VD Model Rats

作　　者：吴红彦 李海龙 杨长生 王虎平 顾静 

机构地区：[1]甘肃省中药新产品创制工程实验室,甘肃兰州730000 [2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000 [3]甘肃省中医方药挖掘与创新转化重点实验室,甘肃兰州730000

出　　处：《中药材》2015年第12期2578-2582,共5页Journal of Chinese Medicinal Materials

基　　金：甘肃省中药药理与毒理学重点实验室自主课题(ZDSYS-ZZKJ-2013(B)-004);国家自然科学基金(81060284)

摘　　要：目的:考察归芪聪志汤对VD模型大鼠的作用及其机制。方法:采用"反复夹闭颈动脉加注射硝普钠降压再加单侧永久结扎颈总动脉法"制备VD大鼠模型,并予归芪聪志汤干预,从行为学、组织病理学、热休克蛋白(HSP)分子水平三个方面考察归芪聪志汤对VD的影响。结果:与假手术组比较,模型组大鼠定位航行潜伏期延长,空间搜索穿越平台次数减少;海马组织细胞数量较少、细胞轮廓模糊皱缩、胞浆深染、核不清晰;HSP27、HSP70mRNA及蛋白相对表达量显著升高。归芪聪志汤中、高剂量能显著改善上述指标,中剂量归芪聪志汤疗效优于脑复康。结论:归芪聪志汤能保护VD大鼠脑组织,改善其病理损伤和记忆功能,其作用机制可能与调节热休克蛋白的表达有关。Objective： To study the effect of Guiqicongzhi Decoction on expression of HSP27 and HSP70 in brain tissue of VD model rats. Methods： The method of＂repeatedly clip carotid artery join with injection of sodium nitroprusside and with permanent unilateral carotid artery ligation＂was used to prepare the vascular dementia model. And then the effect of Guiqicongzhi Decoction on the model rats from rats praxiology,histopathological and the molecules expression of heat shock protein（ HSP） were observed. Results： Compared with control group,the navigation incubation period extended and space search ability became worse in model group; cell number was less,contour fuzzy shrivel,cytoplasm deep stain and nuclear was not clear in hippocampus pathological section; as well as an increase in the expression of HSP27 and HSP70. The above indexes changed significantly in middle dose group and high dose group. The curative effect of middle dose group was better than piracetam. Conclusion： Guiqicongzhi Decoction can protect brain tissue and improve the pathological damage and memory functions of VD rats,the mechauism maybe related to the regulation of HSP27 and HSP70 expression.

关 键 词：血管性痴呆 归芪聪志汤 热休克蛋白 

分 类 号：R285.5[医药卫生—中药学]