当归治疗阿托品诱发小鼠肠蠕动迟缓实验研究  

Effects of Radix Angelica Sinensis in therapy of intestinal hypoperistalsis induced by atropine in mice

作　　者：张彩霞[1] 刘峰林[1,2] 任远[1,2] 马文梅[1] 袁菊丽[1] 王瑞琼[1] 吴国泰[1,2] 

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

出　　处：《中兽医医药杂志》2016年第1期32-34,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省自然科学基金(N0BH2012-122);甘肃省中药药理与毒理学重点实验室2015年度开放基金(NoZDSYS-KJ-2015-008)

摘　　要：目的:观察当归对阿托品诱发小鼠便秘的治疗作用。方法:动物分为对照组、阿托品组、当归低剂量组、当归中剂量组、当归高剂量组,对照组灌胃0.4 m L生理盐水,其余各组以8 mg/kg剂量连续灌胃阿托品4 d,同时以4.8 g/kg、9.6 g/kg、14.4 g/kg剂量连续灌胃当归水煎液4 d,末次给药前12 h停食给水,当归药液、生理盐水均含10%活性炭,给药30 min后,颈椎脱臼处死各组8只小鼠,以炭末推进率、6 h时间内的排粪重量为指标,观察当归对阿托品诱导小鼠肠运动的影响。采用SPSS17.0数据分析软件,应用单因素方差分析SNK进行组间比较。结果:与阿托品组比较,当归中剂量组炭末推进率差异显著(P<0.05),高剂量组差异极显著(P<0.01);与空白组比较,6 h内阿托品组排粪数量、粒数差异不显著;与阿托品组比较,当归中剂量组、当归高剂量组排粪数量、粒数差异不显著,但有促进排粪趋势。结论:当归可以改善阿托品诱发的肠蠕动迟缓,其机制可能为解除阿托品引起的肠肌麻痹。Objective： To determine the therapeutic effect of Angelica Sinensis（AS） on atropine-induced constipation. Methods：Mice were randomly divided into control, atropine group（8 mg/kg）, AS high-dose（14.4 g/kg）, AS medium-dose（9.6 g/kg） and AS lowdose（4.8 g/kg） groups with 8 in each. Control group was given 0.4 m L of normal saline by gavage, other groups were given 8.0 mg/kg of atropine and different dose of AS extract with water for 4 days simultaneously. After food supply were prohibited for 12 h, every tested sample mixed 10% powder of carbon is administrated in last ig, 30 min later, partial mice of every group were executed by removing cervical vertebra for observing charcoal-powder propelling rate, and other mice of every group were recorded to their amount of feces in 6 hours after administration. Results： Compared with atropine group, Charcoal-powder propelling rate was increased in AS highdose group（ASHDG, P〈0.01） and AS medium-dose group（ASMDG, P〈0.05）. The feces amounts in ASHDG and ASMDG had no statistic significance compared with control group. Conclusion： Radix Angelica Sinensis can resist intestinal hypoperistalsis induced by atropine, the mechanism may be abolishment of intestinal muscle paralysis caused by atropine.

关 键 词：当归 阿托品 便秘 肠蠕动 炭末推进率 肠平滑肌麻痹 蠕动迟缓 

分 类 号：S857.3[农业科学—临床兽医学]