替莫唑胺-聚乳酸/羟基乙酸纳米缓释系统对C6胶质瘤细胞凋亡的影响  

Effects of temozolomide-PLGA nanometer sustained-release microsphere on apoptosis of glioma C6 cells

作　　者：林和璞[1,2] 王强[1] 徐维林[1] 张宏伟[1] 马晓东[1] 

机构地区：[1]解放军总医院神经外科,北京100853 [2]北京军区总医院附属八一脑科医院,北京100700

出　　处：《解放军医学院学报》2016年第2期164-166,170,共4页Academic Journal of Chinese PLA Medical School

基　　金：北京市卫生局首都卫生发展科研专项项目基金(2011-5001-08)~~

摘　　要：目的观察替莫唑胺(temozolomide,TMZ)-聚乳酸/羟基乙酸(polylactic acid/glycolic acid,PLGA)缓释微球对胶质瘤细胞活性及凋亡的影响。方法将C6胶质瘤细胞样品分为实验组及空白对照组。试验组加入载药量分别为2.5%、5%、7.5%及10%的替莫唑胺-聚乳酸/羟基乙酸缓释微球,空白对照组仅加入PLGA而不加入任何药物。全自动酶标仪检测细胞活性,多参数流式细胞仪分析细胞凋亡率。结果 2.5%、5%、7.5%及10%实验组在第14天时肿瘤细胞凋亡率分别为30.45%±0.24%、35.87%±0.58%、41.56%±0.82%、43.34%±1.24%,与对照组细胞凋亡率(3.02%±0.24%)相比,均能更有效杀灭C6胶质瘤细胞,并且载药量越高效果越强(P<0.05)。使用MTT法检测各组C6胶质瘤细胞的细胞活性,2.5%、5%、7.5%、10%实验组在第3天时细胞活性分别为23.47%±2.65%、14.58%±5.76%、6.24%±7.32%、4.36%±2.41%,与对照组(99.60%)相比,均能更有效抑制肿瘤细胞活性,且载药量越高效果越强(P<0.05),但7.5%组与10%组差异无统计学意义。结论载药量不同的TMZ-PLGA缓释微球均对C6胶质瘤细胞有杀灭作用,一定范围内,载药量越高的缓释微球抑制胶质瘤细胞活性的作用越强。Objective To observe the effect of temozolomide（TMZ）- polylactic acid/glycolic acid（PLGA） release microspheres（MS） on viability and apoptosis of glioma cell. Methods C6 glioma cell samples were divided into experimental group and control group. TMZ- PLGA release microspheres was added into experimental group with the drug loading of 2.5%, 5%, 7.5% and 10%, and only PLGA was added into blank control group. Cell activity was detected with automatic microplate reader and cell apoptosis rate was analyzed with multi-parameter flow cytometry. Results The apoptosis rate of tumor cell in experimental group with drug loading of 2.5%, 5%, 7.5% and 10% on day 14 were 30.45%±0.24%, 35.87%±0.58%, 41.56%±0.82%, 43.34%±1.24%, respectively. Compared with apoptosis rate in control group（3.02%±0.24%）, it could kill C6 glioma cells effectively, and the effect enhanced with the increasing of drug loading（P〈0.05）. The viability of C6 glioma cells detected using MTT was 23.47%±2.65%, 14.58%±5.76%, 6.24%±7.32% and 4.36%±2.41% in experimental group with drug loading of 2.5%, 5%, 7.5% and 10% on day 3, compared with control group（99.60%）, the tumor cell activity could be inhibited effectively in experimental groups, and the effect enhanced with the increasing of drug loading（P〈0.05）, but there was no significant difference between group of 7.5% and group of 10%. Conclusion The C6 glioma cells can be killed by TMZ-PLGA release microspheres of different degree of drug loading. Within a certain range, the higher the drug loading of release microsphere is, the stronger the effect of inhibiting of glioma cells is.

关 键 词：替莫唑胺 聚乳酸-羟基乙酸共聚物 纳米缓释 

分 类 号：R943[医药卫生—药剂学]