脊髓慢性压迫性损伤后细胞凋亡和BDNF表达的观察  

Observation of cell apoptosis and BDNF expression in spinal cord injured by chronic compression

作　　者：李晓东 徐军[2] 

机构地区：[1]海慈医疗集团神经外科,山东青岛266000 [2]宁夏医科大学总医院心脑血管病医院神经外科,银川750004

出　　处：《中国临床神经外科杂志》2016年第3期167-170,共4页Chinese Journal of Clinical Neurosurgery

基　　金：宁夏回族自治区自然科学基金(NZ11200)

摘　　要：目的探讨大鼠脊髓在遭受到持续进行性压迫损伤后神经细胞凋亡以及脑源性神经营养因子（BDNF）及其Trk B受体的变化。方法将75只SD大鼠分为模型组、对照组和正常组,各25只;根据造模后取材时间,各组再分为1、7、14、21、28 d 5亚组,每亚组5只。胸11～12椎板和硬脊膜之间置入缓膨材料（3 mm×5 mm,厚0.8 mm）制作大鼠慢性压迫性脊髓损伤模型,BBB评分评估行为学变化,TUNEL染色检测细胞凋亡,免疫组化染色检测BDNF及其Trk B受体表达变化。结果造模后7、14、21、28 d,模型组大鼠BBB评分均明显低于对照组和正常组（P〈0.05）,而对照组和正常组均无统计学差异（P〉0.05）。模型组大鼠可观察到从脊髓受压开始,神经细胞开始出现凋亡,中央管及前角区域的神经细胞凋亡明显,邻近灰质的白质部分神经胶质细胞凋亡明显;而对照组和正常大鼠未见明显凋亡细胞。模型组大鼠脊髓内BDNF及其Trk B受体呈强阳性,尤其是神经元部位,BDNF及其受体Trk B表达明显,且主要表达在运动类神经元中,随压迫进行,表达逐渐增强,至相对稳定;对照组和正常组大鼠脊髓内BDNF及其Trk B受体表达较少。结论大鼠脊髓在受到慢性压迫性损伤时,神经细胞凋亡明显,BDNF、Trk B受体表达明显增强。Objective To explore the pathogenetic mechanism of injury to spinal cord caused by chronic compression.Methods Seventy-five SD rats were divided into three groups of 25 animal each, i.e. normal group, experimental group, in which the model of spinal cord injury was caused by the chronic compression and control group which was similar to the experimental group in surgical treatment except the chronic compression of the spinal cord. The spinal cord function was determined by Basso, Beattie and Bresnahan locomotor rating scale（BBB Scale） postoperatively 1, 7, 14, 21 and 28 days after the pressure issues in all the groups, in which cells apoptosis and the expression of brain derived neurotrophic factor（BDNF） and its receptor, tropomyosin receptor kinase B（Trk B） in theinjured spinal cord were determined respectively by Td T-mediated du TP nick-end labeling（TUNEL） and immunohistochemical staining28 days after the pressure issues.Results BBB scores were significantly lower in the experimental group than those in the other twogroups 1, 7, 14, 21 and 28 days after the pressure issues（P〈0.05）. TUNEL staining showed that there were apoptosis of many cells in the injured spinal cord tissues in the experimental group and no cells apoptosis was found in the other two groups. The immunohistochemical staining showed that the expressions of BDNF and its receptor Trk B were stronger in the spinal cord tissues in the experimental groupthan those in the other two groups.Conclusion Apoptosis and the expression of BDNF, Trk B in the spinal cord injured by chroniccompression, which can cause significant injury to the spinal cord function, are significantly enhanced.

关 键 词：慢性脊髓压迫性损伤 脑源性神经营养因子 TRK B受体 细胞凋亡 大鼠 

分 类 号：R651.2[医药卫生—外科学] R363.21[医药卫生—临床医学]