戈登菌mmpL3同源基因序列分析及功能预测  

Sequencing of a homologue of mmpL3 from Gordonia bronchialis and prediction of its function

作　　者：陈高瞻[1] 孙妍[1] 占卫红 雷航[1] 王心倩 宋言峥 张舒林[3] 余晓丽[1] 

机构地区：[1]武汉轻工大学生物与制药工程学院,湖北武汉430023 [2]上海公共卫生临床中心,上海201508 [3]上海交通大学医学院免疫与微生物学系,上海200025

出　　处：《中国病原生物学杂志》2016年第2期147-150,共4页Journal of Pathogen Biology

基　　金：武汉轻工大学研究生创新基金项目(No.2014cx019)

摘　　要：目的分析支气管戈登菌临床株mmpL3(Rv0206c)同源基因(Gbro4481)序列,并预测其蛋白质结构及功能,为Gbro4481蛋白的进一步研究奠定基础。方法根据全基因组测序结果和同源比对找到支气管戈登菌的mmpL3同源基因,应用expasy工具预测支气管戈登菌MmpL3同源蛋白理化性质;利用Interproscan和Gene ontology工具对蛋白保守序列和功能进行预测;使用TMHMM在线工具进行拓扑结构预测。结果筛选出MmpL3同源蛋白(Gbro4481),该蛋白含有2个膜转运蛋白结构域和1个固醇敏感多肽区;TMHMM预测Gbro4481蛋白有10个跨膜区。结论支气管戈登菌的Gbro4481蛋白与结核分枝杆菌H37Rv的mmpL3蛋白同源,是分枝菌酸的转运蛋白,参与胞内小分子的运输和信号转导。Objectives To analyze the physicochemical characteristics and to predict the functions of Gbro4481,a homologue of MmpL3 from Mycobacterium tuberculosis,from Gordonia bronchialis in order to provide evidence for further study of the structure and function of Gbro4481 in G.bronchialis. Methods G.bronchialis isolates were screened for homologues of mmpL3 based on genomic sequencing and analysis of the homology of G.bronchialis strains.The physicochemical properties of the homologue were predicted with expasy,conserved domains of the protein were predicted with Interproscan,and the functions of the protein were predicted with Gene ontology.The Web-based programs PSORTb,SignalP,and TMHMM were used to analyze the subcellular localization,signal peptides,and the topology of the protein. Results A protein homologous to MmpL3 was identified.This protein,Gbro4481,has two MMPL domains and a sterol-sensing domain.Gbro4481 was calculated to have a molecular weight of 116,051.2and was estimated to have a pI of8.94.According to predictions,the protein would be expressed in the cell membrane and have no signal peptides.There are ten transmembrane domains in this membrane protein. Conclusion Gbro4481 fromG.bronchialisis homologous to mmpL3 from M.tuberculosis,and is presumably involved in lipid metabolism,signal transduction,and substance transport.

关 键 词：支气管戈登菌 mmpL3同源基因 生物信息学分析 

分 类 号：R378.91[医药卫生—病原生物学]