HPLC法测定不同主产地黄芪饮片的4种黄酮的含量  

Determination of Four Flavonoids in Astragalus Pieces of Different Main Origins by HPLC

作　　者：蒲清荣 黄锐 赵剑 明俊男 罗群 李静 

机构地区：[1]四川医科大学附属中医医院,四川泸州646000

出　　处：《云南中医中药杂志》2016年第3期63-65,共3页Yunnan Journal of Traditional Chinese Medicine and Materia Medica

基　　金：四川省科技厅项目(NO:2013SZ0143)

摘　　要：目的采用HPLC法同时测定不同主产地黄芪饮片中毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素的含量。方法色谱柱固定相为Inertsil ODS-SP C18柱（250 mm×4.6 mm,5μm）,流动相乙腈-水（含0.2%甲酸）,梯度洗脱,流速1.0 m L·min-1,紫外检测波长260 nm,柱温为40℃。结果毛蕊异黄酮葡萄糖苷、芒柄花苷、毛蕊异黄酮、芒柄花素在0.00569~0.0569、0.00448~0.0448、0.00257~0.0257、0.00265~0.0265mg/m L时与色谱峰面积呈良好的线性关系,平均回收率为98.72%、99.32%、100.03%、99.78%,RSD为1.06%、2.32%、2.86%、1.08%。结论该方法准确灵敏,可作为评价不同产地黄芪饮片的质量控制方法。Objective： To determine the contents of calycosin- glucoside,ononin,calycosin and formononetin in Astragalus Pieces of different main origins by HPLC. Methods： Column stationary phase was Inertsil ODS- SP C18（ 250 mm × 4. 6 mm,5 μm） and mobile phase was acetonitrile- water（ containing 0. 2% formic acid）,by gradient elute,with 1. 0 m L·min- 1of flow rate and 260 nm of ultraviolet detector wavelength,at 40℃ of column temperature. Results： The calycosin- glucoside,ononin,calycosin and formononetin showed a good linear relationship with chromatographic peak areas at the range of 0. 00569- 0. 0569,0. 00448- 0. 0448,0. 00257- 0. 0257 and0. 00265- 0. 0265 mg / m L. The average recovery was 98. 72%,99. 32%,100. 03% and 99. 78%,and RSD was 1. 06%,2. 32%,2. 86% and 1. 08%. Conclusion： The method is accurate and sensitive and can be valued as a quality control method for Astragalus Pieces of different origins.

关 键 词：黄芪饮片 毛蕊异黄酮葡萄糖苷 芒柄花苷 毛蕊异黄酮 芒柄花素 HPLC 

分 类 号：R284.2[医药卫生—中药学]