大孔吸附树脂纯化黄花柳花总黄酮工艺研究  

Purification of Total Flavonoids From the Flowers of Salix caprea L. by the Macroporous Adsorption Resin

作　　者：方贺[1] 宋旺弟 陈文[1] 

机构地区：[1]石河子大学药学院,新疆石河子832000

出　　处：《北方园艺》2016年第6期120-124,共5页Northern Horticulture

基　　金：国家"重大新药创制"科技重大专项资助项目(2011ZX09401-007)

摘　　要：以黄花柳花为试材,采用紫外分光光度法,选择5种不同型号的树脂进行静态吸附试验,筛选出对其吸附解吸效性能高的树脂,再通过动态吸附与解吸试验研究上样浓度、树脂上样量、洗脱剂及流速等条件对黄花柳花总黄酮纯化效果的影响,优化维药黄花柳花总黄酮的大孔树脂纯化工艺。结果表明:AB-8型大孔吸附树脂对黄花柳花总黄酮具有较好的吸附解吸效果,最佳纯化条件为黄花柳花待纯化液浓度为3.13g/L,调节上样液pH 4.2,上样量5.5mL/g,以1.0mL/min流速上样,依次用10BV蒸馏水淋洗,6BV 70%乙醇以2.0mL/min流速洗脱,得纯化液。经AB-8纯化后的黄花柳花总黄酮纯度达51.99%。AB-8型大孔吸附树脂能有效提高黄花柳花中总黄酮纯度,并符合中药有效部位研究要求。Taking Salix caprea L.as material,the optimum technological parameters of the purification process of total flavones fromSalix caprea L.flowers with macroreticular resins was explored.The contents of total flavonoids were determined by using UV-Vis spectrum.Five kinds of resins were examined by static test.Purification conditions were investigated by using dynamic adsorption and desorption experiment.The results showed that AB-8macroporus resin had good adsorption and desorption properties for favonoids in Salix capreaflowers.The optimal refined conditions were as follows：the velocity of sample solution 1.0mL/min and pH 4.2,sample concentration of 3.13g/L,the loaded amount of5.5mL/g,washing with distilled water 10 BV,then eluted with 6BV of 70% ethanol at 2.0mL/min.Under those optimum conditions,the purity of total flavonoids and the recovery was up to 51.99%.AB-8macroreticular absorptive resin could effectively separate and purify total flavones fromSalix capreaflowers,the purity coefficient thus was over50%,which was in line with the study of effective components of herbal medicine.

关 键 词：黄花柳花 大孔树脂 总黄酮 纯化工艺 

分 类 号：R284.1[医药卫生—中药学]