油菜花粉总脂肪酸部位的提取与纯化工艺研究  

Extraction and purification process of total fatty acid in Brassica campestris pollen

作　　者：李璐[1,2] 吴云[1,2] 原少伟[1,2] 杨晶[1,2] 杨义芳[3] 丁岗[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中国中药杂志》2016年第2期226-232,共7页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-007);上海市自然科学基金项目(06ZR14078);上海市中药现代化专项项目(08DZ1971801;09DZ1975200)

摘　　要：对油菜花粉总脂肪酸部位的提取(CO2超临界萃取)和纯化(醇洗除杂)工艺进行优化。通过星点设计-响应面法,以脂肪油的得率为指标,对油菜花粉总脂肪酸部位的CO2超临界萃取工艺进行了优化;通过正交设计,以总脂肪酸(亚麻酸酰胺、亚麻酸甘油酯、亚麻酸和棕榈酸)含量为指标,对提取物进行醇洗除杂。优化得到超临界萃取工艺为萃取温度60℃,萃取压力35 MPa,萃取时间3 h,纯化工艺为醇浓度80%,醇倍量为50倍,醇提时间1.5 h。油菜花粉总脂肪酸部位经提取纯化,质量分数能达到60%左右,且该工艺稳定可行,适于工业生产。This study was aimed to optimize the extraction（CO2supercritical extraction） process and purification（ ethanol washing and decontaminating） process of total fatty acid in Brassica campestris pollen. With the extraction yield of total fatty acid as index,CO2 supercritical extraction of total fatty acid in B. campestris polle was optimized by central composite design-response surface methodology. Ethanol washing and decontaminating was done for the extract through orthogonal design with content of total fatty acid（ linolenic acid amide,linolenic acid glyceride,linolenic acid and palmitic acid） as the indexes. The optimum parameters of CO2 supercritical extraction technology were as follows： extraction pressure of 35 MPa,extraction temperature of 60 ℃ and extraction time of 3 h. When extract of supercritical fluid was purified by 50 times of 80% ethanol for 1. 5 h,the content of total fatty acid can reach 60%. In addition,this process was stable and steady,provided reliable basis for production.

关 键 词：超临界萃取 油菜花粉 总脂肪酸 星点设计 响应面法 

分 类 号：R284.2[医药卫生—中药学]