应用自动质谱退卷积定性系统(AMDIS)和保留指数分析3种不同基原莪术的挥发油成分差异  

Study on composition of essential oils in three different origins' Curcumae Rhizoma by AMDIS and retention index

作　　者：郑勇凤[1] 汪蕾[1] 赵思蕾 甘彦雄 余佳丽[1] 廖婉[1] 傅超美[1] 

机构地区：[1]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中国中药杂志》2016年第2期257-263,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金青年项目(81403103);中药资源四川省青年科技创新研究团队项目(2015TD0028);四川省科技支撑计划(2014SZ0156);四川省教育厅面上项目(2013SZB0781)

摘　　要：对比分析不同基原、不同产地莪术的挥发油成分差异。采用GC-MS技术和自动质谱退卷积定性系统(AMDIS),分析《中国药典》2010年版收载的3种基原莪术的挥发油总离子流色谱图。具体步骤为:应用AMDIS拆分重叠峰,通过NIST11.L标准质谱库检索,结合程序升温保留指数PTRI辅助定性;通过选择特征性碎片离子峰确定各拆分峰对应的峰面积,采用峰面积归一化法确定各化合物的相对百分含量。结果显示不同基原、不同产地的莪术挥发油成分的化合物种类及相对百分含量存在不同程度的差异,提示莪术挥发油成分可在一定程度上作为评价不同基原莪术质量及道地性特征的指标。To compare the differences of essential oils extracted from Curcumae Rhizoma with different origins. The TIC of the essential oils of Curcumae Rhizoma from three different origins recorded by CP（2010）,were investigated by GC-MS combined with automated mass spectral deconvolution and identification system（ AMDIS）,steps as follow： firstly,overlapped peaks were resolved by AMDIS,secondly,NIST11. L standard MS spectral database combined with retention index were used to assist qualitative analysis,thirdly,the peak area of each split peak were determined by choosing the characteristic fragment ion peak,finally,the relative percentage contents of each compounds were determined through peak area normalization method. The results showed that the components of the essential oils of Curcumae Rhizoma from three origins were different from each other at various degree. In conclusion,the types and content of essential oils could be an indicator to evaluate the quality of Curcumae Rhizoma from different origins and its geoherbalism.

关 键 词：莪术 GC-MS-AMDIS 保留指数 挥发油 定性定量分析 

分 类 号：R284[医药卫生—中药学]