金属硫蛋白对皮肤癌发生抵抗作用及机制的研究  

Antagonizing effects of Metallothionein against development of skin cancer and relevant mechanisms

作　　者：武月婷[1] 时佳宏[1] 潘奇正[2] 王元[1] 侯宝莲 任淑萍[1] 

机构地区：[1]吉林大学公共卫生学院劳动卫生与环境卫生教研室,长春130021 [2]吉林大学中日联谊医院,长春130000

出　　处：《中国免疫学杂志》2016年第3期340-344,共5页Chinese Journal of Immunology

摘　　要：目的:建立Ha Ca T细胞UVB损伤模型,给予硫酸锌刺激,探讨其对细胞损伤的拮抗作用及机制。方法:细胞分为正常组、加锌组、紫外线组、加锌紫外线组,照射前24 h给予硫酸锌处理,UVB照射后继续培养,确定硫酸锌给药浓度和UVB辐射时间,Western blot法检测细胞凋亡情况,免疫细胞化学方法检测金属硫蛋白和NF-κB/p65的表达情况。结果:紫外线组细胞内Bax/Bcl-2比值高于正常组和加锌紫外线组;紫外线组、加锌组细胞内MT表达水平均高于正常组,加锌紫外线组细胞MT表达水平高于紫外线组;紫外线组细胞内NF-κB/p65表达水平高于正常组和加锌紫外线组细胞。结论:硫酸锌能够通过细胞内金属硫蛋白的表达,缓解UVB诱发Ha Ca T细胞凋亡的发生。Objective： To establish a UVB damage cell model with Ha Ca T cells to investigate the protective effects of Zinc sulfate on the cell damage caused by UVB and its relevant mechanisms. Methods： The cells were divided into normal group,Zinc group,UVB group,Znic and UVB group. The addition of Zinc sulfate to the Ha Ca T cells was conducted 24 h prior to the irradiation to the cells by UVB. Cell apoptosis was detected by Western blot and the expression of metallothionein and NF-κB/p65 were measured by immunohistochemistry. Results： Compared with normal and Zn ＋ UVB group,Bax/Bcl-2 rate in UVB group increased. Compared with normal group,MT expression levels in UVB group,Zn group increased,and compared with UVB group,MT expression level in Zn＋UVB group increased. Compared with normal group and Zn＋UVB group,NF-κB/p65 expression level in UVB group increased. Conclusion： Zinc sulfate alleviates the apoptosis of Ha Ca T cell induced by UVB because of the expression of MT.

关 键 词：皮肤癌 紫外线 金属硫蛋白 细胞凋亡 

分 类 号：R392[医药卫生—免疫学]